STUDIES ON GENETIC CONTROL OF LOW-MOLECULAR-WEIGHT GLUTENIN SUBUNITS IN WHEAT ENDOSPERM BY A-PAGE ANALYSIS

Genetic control of low-molecular-weight glutenin subunits (LMW-GS) of nullisomictetrasomic (NT) and ditelocentric (DT) lines of Chinese Spring, and substitution lines of Cheyenne-Wichiita were investigated using acid-polyacrylamide gel electrophoresis (APAGE) analysis. The results showed that genes...

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Bibliographic Details
Published in:Cereal research communications 1999-01, Vol.27 (3), p.251-257
Main Authors: Yan, Y., Prodanovic, S., Mladenov, N., Milovanovic, M.
Format: Article
Language:English
Subjects:
Alleles
Centrifugation
Chromosomes
Electrophoresis
Endosperm
Gels
Genes
Genetic loci
Genetics
Hybridity
Wheat
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Summary:Genetic control of low-molecular-weight glutenin subunits (LMW-GS) of nullisomictetrasomic (NT) and ditelocentric (DT) lines of Chinese Spring, and substitution lines of Cheyenne-Wichiita were investigated using acid-polyacrylamide gel electrophoresis (APAGE) analysis. The results showed that genes coding for about 20 LMW-GS could be localized on the short arms (Glu-3 loci) of homologous group 1 chromosomes. On the basis of linkages between Gli-1 loci and Glu-3 loci, several LMW-GS were showed to be coded by different Glu-3 loci by the analysis of F3 generation of Sana x Francuska hybrid. Of 8 LMW glutenin subunits analyzed, 2, 3 and 3 LMW-GS were controlled by Glu-A3, Glu-B3 and GluD3 locus, respectively. A-PAGE was capable of rapid, high-resolution separation of glutenin subunits. This system, thus, may become a more efficient alternative to routine SDS-PAGE for investigating composition and genetic control of wheat glutenin subunits.
ISSN:0133-3720
1788-9170
DOI:10.1007/bf03543533