An improved method for isolating violet root rot fungus, Helicobasidium mompa, from basidiocarps

Basidiocarps of the violet root rot fungus, Helicobasidium mompa, are less frequently used for isolation than are mycelial strands and sclerotia even though the basidiocarps are conspicuously produced at the trunk base of diseased plants. Basidiocarps are also more suitable for storage. This paper d...

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Bibliographic Details
Published in:Journal of general plant pathology : JGPP 2001-02, Vol.67 (1), p.37-40
Main Authors: Nakamura, H. (National Inst. of Agro-Environmental Sciences, Tsukuba, Ibaraki (Japan)), Uetake, Y, Arakawa, M, Okabe, I, Matsumoto, N
Format: Article
Language:English
Subjects:
CULTURE TECHNIQUES
FUNGAL SPORES
HELICOBASIDIUM
ISOLATION
LABORATORY EXPERIMENTATION
LIRIODENDRON TULIPIFERA
SPOROPHORES
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Summary:Basidiocarps of the violet root rot fungus, Helicobasidium mompa, are less frequently used for isolation than are mycelial strands and sclerotia even though the basidiocarps are conspicuously produced at the trunk base of diseased plants. Basidiocarps are also more suitable for storage. This paper describes an improved method for obtaining pure cultures from basidiocarps using microcentrifuge tubes to facilitate the awkward steps of rinsing fungal materials under a dissecting microscope.
ISSN:1345-2630
1610-739X
DOI:10.1007/PL00012984