Inoculation of Lupinus luteus with white root rot fungus, Rosellinia necatrix, to estimate virulence

An inoculation method using Lupinus luteus was developed for estimating virulence of isolates of the white root rot fungus, Rosellinia necatrix Prillieux. Fungal cultures grown on pieces of mulberry twigs were placed in contact with the hypocotyl of 3-week-old seedlings growing in pots of soil. Dise...

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Bibliographic Details
Published in:Journal of general plant pathology : JGPP 2001-11, Vol.67 (4), p.285-287
Main Authors: Uetake, Y. (National Inst. for Agro-Environmental Sciences, Tsukuba, Ibaraki (Japan)), Nakamura, H, Arakawa, M, Okabe, I, Matsumoto, N
Format: Article
Language:English
Subjects:
EVALUATION
INOCULATION
LUPINUS LUTEUS
ROOT ROTS
ROSELLINIA
TOXICITY
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Summary:An inoculation method using Lupinus luteus was developed for estimating virulence of isolates of the white root rot fungus, Rosellinia necatrix Prillieux. Fungal cultures grown on pieces of mulberry twigs were placed in contact with the hypocotyl of 3-week-old seedlings growing in pots of soil. Disease development was uniform and reproducible in repeated experiments. Of 24 isolates with double-stranded RNA, eight were weakly virulent. This method is useful throughout the year for estimating virulence of many isolates of the fungus and for screening for hypovirulent isolates.
ISSN:1345-2630
1610-739X
DOI:10.1007/PL00013031