A high-throughput screening method for GM soybean events based on single universal primer multiplex PCR and capillary electrophoresis

• Published in: Journal für Verbraucherschutz und Lebensmittelsicherheit 2024-12, Vol.19 (4), p.445-454
• Main Authors: Dong, Liming, Li, Congcong, Xing, Zhenjuan, He, Yuxuan, Xia, Wei, Liu, Yijun, Long, Likun, Li, Feiwu
• Format: Article
• Language: English
• Subjects: Agriculture; Biomedical and Life Sciences; Biotechnology; Chemistry/Food Science; Food Science; Life Sciences; Methods; Plant Genetics and Genomics
• ISSN: 1661-5751; 1661-5867
• DOI: 10.1007/s00003-024-01524-6

Genetically modified (GM) crops and their products are regulated in many countries. To meet increasing regulatory requirements for a growing number of GM soybean events, high-throughput and cost-effective detection methods are indispensable. In this study, 14 widely commercialized GM soybean events, including GTS40-3-2, MON89788, CV127, A2704-12, A5547-127, 305423, 356043, MON87701, MON87705, MON87708, MON87769, SYHT0H2, FG72, and DAS-444Ø6-6 were utilized as targets for event-specific identification. A high-throughput detection method was established by integrating a single universal primer multiplex PCR (SUP-M-PCR) with capillary electrophoresis technique. This method enables the identification of 14 soybean events in a single PCR reaction, addressing the limitations of conventional multiplex PCR such as self-inhibition and amplification variations between different primers. Furthermore, it significantly enhances the sensitivity and accuracy of previous multiple detection methods, achieving a detection sensitivity of 0.05% (w/w). Overall, this approach greatly improves detection efficiency and holds promising potential for the identification and detection of 14 types of GM soybean events and their derivatives.