Toxicity of fungicides containing ethylene-bis-dithiocarbamate in serumless dissociated mesencephalic-striatal primary coculture

Agricultural exposure to the organomanganese fungicide MANEB (manganese-ethylene-bis-dithiocarbamate) may induce an extrapyramidal syndrome resembling parkinsonism. To evaluate the relative role of manganese (Mn) and ethylene-bis-dithiocarbamate (EBDTC) in the hazard of organomanganese fungicides, w...

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Bibliographic Details
Published in:Archives of toxicology 1996, Vol.70 (10), p.678-682
Main Authors: SOLEO, L, DEFAZIO, G, SCARSELLI, R, ZEFFERINO, R, LIVREA, P, FOA, V
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Coculture Techniques
Corpus Striatum - cytology
Corpus Striatum - drug effects
Culture Media, Serum-Free
Ethylenebis(dithiocarbamates) - toxicity
Fungicides, Industrial - toxicity
Maneb - toxicity
Medical sciences
Mesencephalon - cytology
Mesencephalon - drug effects
Pesticides, fertilizers and other agrochemicals toxicology
Rats
Toxicology
Zineb - toxicity
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Summary:Agricultural exposure to the organomanganese fungicide MANEB (manganese-ethylene-bis-dithiocarbamate) may induce an extrapyramidal syndrome resembling parkinsonism. To evaluate the relative role of manganese (Mn) and ethylene-bis-dithiocarbamate (EBDTC) in the hazard of organomanganese fungicides, we studied the effects of MANCOZEB (Mn-Zinc-EBDTC) and ZINEB (Zinc-EBDTC) on serumless dissociated mesencephalic-striatal primary coculture. High affinity 3H-dopamine (DA) and 14C GABA uptakes as well as immunocytochemical staining of tyrosine hydroxylase (TH)-containing cells were used as specific functional markers of DA and GABA neuron viability. Both MANCOZEB and ZINEB, at 10 and 50 microM concentrations, dose dependently reduced DA and GABA viability parameters. These data suggest that EBDTC rather than Mn may be primarily responsible for the cytotoxicity of organomanganese fungicides on neuronal systems relevant to the pathophysiology of parkinsonism.
ISSN:0340-5761
1432-0738
DOI:10.1007/s002040050328