Modulation of butyrate transport in Caco-2 cells

• Published in: Naunyn-Schmiedeberg's archives of pharmacology 2009-04, Vol.379 (4), p.325-336
• Main Authors: Gonçalves, Pedro, Araújo, João Ricardo, Pinho, Maria João, Martel, Fátima
• Format: Article
• Language: English
• Subjects: 2,4-Dinitrophenol - pharmacology; 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid - pharmacology; 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - pharmacology; Acetaldehyde - pharmacology; Acids, Acyclic - pharmacology; Aspirin - pharmacology; Biological Transport, Active - drug effects; Biomedical and Life Sciences; Biomedicine; Butyrates - metabolism; Caco-2 Cells; Caffeine - pharmacology; Cell Survival - drug effects; Dronabinol - pharmacology; Gene Expression - drug effects; Gene Expression - genetics; Humans; Hydrogen-Ion Concentration; Indomethacin - pharmacology; Ions - pharmacology; Kinetics; Monocarboxylic Acid Transporters - antagonists & inhibitors; Monocarboxylic Acid Transporters - genetics; N-Methyl-3,4-methylenedioxyamphetamine - pharmacology; Neurosciences; Original Article; Pharmacology/Toxicology; Sodium Azide - pharmacology; Symporters - antagonists & inhibitors; Symporters - genetics; Theophylline - pharmacology
• ISSN: 0028-1298; 1432-1912
• DOI: 10.1007/s00210-008-0372-x

The aim of this study was to investigate the putative influence of some pharmacological agents and drugs of abuse upon the apical uptake of butyrate (BT) into Caco-2 cells. The apical uptake of 14 C-BT by Caco-2 cells was (1) time and concentration dependent, (2) pH dependent, (3) Na + independent and Cl − dependent, (4) energy dependent, (5) inhibited by several BT structural analogues (acetate, propionate, α-ketobutyrate, pyruvate, lactate), (6) insensitive to the anion exchange inhibitors DIDS and SITS and (7) inhibited by the monocarboxylate transport (MCT) inhibitors NPPB and pCMB. These characteristics are compatible with an involvement of MCT1-mediated transport. Acutely, uptake of a low concentration of 14 C-BT (10 μM) was reduced by acetaldehyde, acetylsalicylic acid, indomethacin, caffeine and theophylline and increased by MDMA. Chronically, uptake was increased by caffeine and decreased by tetrahydrocannabinol and MDMA; reverse transcription quantitative real-time PCR analysis showed that these three compounds decreased the mRNA levels of MCT1. Acutely, acetaldehyde, indomethacin and MDMA reduced the uptake of a high concentration of 14 C-BT (20 mM), and acetylsalicylic acid increased it. Chronically, none of the compounds affected uptake. Acetaldehyde, indomethacin and propionate seem to be competitive inhibitors of 14 C-BT uptake. Acetylsalicylic acid simultaneously increased the K m and the V max of 14 C-BT uptake. In conclusion, MCT1-mediated transport of 14 C-BT in Caco-2 cells is modulated by either acute or chronic exposure to some pharmacological agents and drugs of abuse (acetaldehyde, acetylsalicylic acid, indomethacin, caffeine, theophylline and the drugs of abuse tetrahydrocannabinol and MDMA).