Adenine attenuates the Ca2+ contraction-signaling pathway via adenine receptor-mediated signaling in rat vascular smooth muscle cells

Our previous study demonstrated that adenine (6-amino-6 H -purine) relaxed contracted rat aorta rings in an endothelial-independent manner. Although adenine receptors (AdeRs) are expressed in diverse tissues, aortic AdeR expression has not been ascertained. Thus, the aims of this study were to clari...

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Published in:Naunyn-Schmiedeberg's archives of pharmacology 2016-09, Vol.389 (9), p.999-1007
Main Authors: Fukuda, Toshihiko, Kuroda, Takahiro, Kono, Miki, Hyoguchi, Mai, Tajiri, Satoshi, Tanaka, Mitsuru, Mine, Yoshinori, Matsui, Toshiro
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Neurosciences
Original Article
Pharmacology/Toxicology
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Summary:Our previous study demonstrated that adenine (6-amino-6 H -purine) relaxed contracted rat aorta rings in an endothelial-independent manner. Although adenine receptors (AdeRs) are expressed in diverse tissues, aortic AdeR expression has not been ascertained. Thus, the aims of this study were to clarify the expression of AdeR in rat vascular smooth muscle cells (VSMCs) and to investigate the adenine-induced vasorelaxation mechanism(s). VSMCs were isolated from 8-week-old male Wistar-Kyoto rats and used in this study. Phosphorylation of myosin light chain (p-MLC) was measured by western blot. AdeR mRNA was detected by RT-PCR. Intracellular Ca 2+ concentration ([Ca 2+ ] i ) was measured by using Fura-2/AM. Vasorelaxant adenine (10–100 μM) significantly reduced p-MLC by angiotensin II (Ang II, 10 μM) in VSMCs ( P  
ISSN:0028-1298
1432-1912
DOI:10.1007/s00210-016-1264-0