Pharmacological and behavioral profile of N-[(3R)-1-azabicyclo[2.2.2]oct-3-yl]-6-chinolincarboxamide (EVP-5141), a novel α7 nicotinic acetylcholine receptor agonist/serotonin 5-HT3 receptor antagonist

• Published in: Psychopharmacology 2013-05, Vol.227 (1), p.1-17
• Main Authors: Boess, Frank G., de Vry, Jean, Erb, Christina, Flessner, Timo, Hendrix, Martin, Luithle, Joachim, Methfessel, Christoph, Schnizler, Katrin, van der Staay, F. Josef, van Kampen, Marja, Wiese, Welf-Burkhard, König, Gerhard
• Format: Article
• Language: English
• Subjects: alpha7 Nicotinic Acetylcholine Receptor - metabolism; Animals; Avoidance Learning - drug effects; Avoidance Learning - physiology; Biomedical and Life Sciences; Biomedicine; Dose-Response Relationship, Drug; Female; HEK293 Cells; Humans; Male; Memory - drug effects; Memory - physiology; Mice; Mice, Inbred C57BL; Neurosciences; Nicotinic Agonists - chemistry; Nicotinic Agonists - metabolism; Nicotinic Agonists - pharmacology; Original Investigation; Pharmacology/Toxicology; Protein Binding - physiology; Psychiatry; Quinolines - chemistry; Quinolines - metabolism; Quinolines - pharmacology; Quinuclidines - chemistry; Quinuclidines - metabolism; Quinuclidines - pharmacology; Rats; Rats, Wistar; Serotonin 5-HT3 Receptor Antagonists - metabolism; Serotonin 5-HT3 Receptor Antagonists - pharmacology; Xenopus laevis
• ISSN: 0033-3158; 1432-2072
• DOI: 10.1007/s00213-012-2933-4

Rationale and objective Agonists of α7 nicotinic acetylcholine receptors (nAChRs) may have therapeutic potential for the treatment of cognitive deficits. This study describes the in vitro pharmacology of the novel α7 nAChR agonist/serotonin 5-HT 3 receptor (5-HT 3 R) antagonist N -[(3 R )-1-azabicyclo[2.2.2]oct-3-yl]-6-chinolincarboxamide (EVP-5141) and its behavioral effects. Results EVP-5141 bound to α7 nAChRs in rat brain membranes ( K i  = 270 nM) and to recombinant human serotonin 5-HT 3 Rs ( K i  = 880 nM) but had low affinity for α4β2 nAChRs ( K i  > 100 μM). EVP-5141 was a potent agonist at recombinant rat and human α7 nAChRs expressed in Xenopus oocytes. EVP-5141 acted as 5-HT 3 R antagonist but did not block α3β4, α4β2, and muscle nAChRs. Rats trained to discriminate nicotine from vehicle did not generalize to EVP-5141 (0.3–30 mg kg −1 , p.o.), suggesting that the nicotine cue is not mediated by the α7 nAChR and that EVP-5141 may not share the abuse liability of nicotine. EVP-5141 (0.3–3 mg kg −1 ) improved performance in the rat social recognition test. EVP-5141 (0.3 mg kg −1 , p.o.) ameliorated scopolamine-induced retention deficits in the passive avoidance task in rats. EVP-5141 (1 mg kg −1 , i.p.) improved spatial working memory of aged (26- to 32-month-old) rats in a water maze repeated acquisition task. In addition, EVP-5141 improved both object and social recognition memory in mice (0.3 mg kg −1 , p.o.). Conclusions EVP-5141 improved performance in several learning and memory tests in both rats and mice, supporting the hypothesis that α7 nAChR agonists may provide a novel therapeutic strategy for the treatment of cognitive deficits in Alzheimer's disease or schizophrenia.