Direct determination of phosphate sugars in biological material by 1H high-resolution magic-angle-spinning NMR spectroscopy

• Published in: Analytical and bioanalytical chemistry 2016, Vol.408 (20), p.5651-5656
• Main Authors: Diserens, Gaëlle, Vermathen, Martina, Gjuroski, Ilche, Eggimann, Sandra, Precht, Christina, Boesch, Chris, Vermathen, Peter
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Biochemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Food Science; Laboratory Medicine; Monitoring/Environmental Analysis; Research Paper
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-016-9671-0

The study aim was to unambiguously assign nucleotide sugars, mainly UDP-X that are known to be important in glycosylation processes as sugar donors, and glucose-phosphates that are important intermediate metabolites for storage and transfer of energy directly in spectra of intact cells, as well as in skeletal muscle biopsies by 1 H high-resolution magic-angle-spinning (HR-MAS) NMR. The results demonstrate that sugar phosphates can be determined quickly and non-destructively in cells and biopsies by HR-MAS, which may prove valuable considering the importance of phosphate sugars in cell metabolism for nucleic acid synthesis. As proof of principle, an example of phosphate-sugar reaction and degradation kinetics after unfreezing the sample is shown for a cardiac muscle, suggesting the possibility to follow by HR-MAS NMR some metabolic pathways. Graphical abstract Glucose-phosphate sugars (Glc-1P and Glc-6P) detected in muscle by 1H HR-MAS NMR