Thermal resistance of tomato polygalacturonase and pectinmethylesterase at physiological pH

The kinetic parameters of heat-induced inactivation of tomato fruit pectic enzymes in a partially purified form have been calculated. Polygalacturonase I (PGI), polygalacturonase II (PGII) and pectinmethylesterase (PME) were separated by means of gel permeation chromatography and their thermoresista...

Full description

Saved in:
Bibliographic Details
Published in:Zeitschrift für Lebensmittel-Untersuchung und -Forschung. A, European food research and technology European food research and technology, 1997-01, Vol.204 (2), p.146-150
Main Authors: Lopez, P, Sanchez, A.C, Vercet, A, Burgos, J
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Biological and medical sciences
CHROMATOGRAPHIE
CHROMATOGRAPHY
CROMATOGRAFIA
ENZYMIC ACTIVITY
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
HEAT TREATMENT
PECTINESTERASAS
PECTINESTERASE
POLIGALACTURONASA
POLYGALACTURONASE
RESISTANCE A LA TEMPERATURE
RESISTENCIA A LA TEMPERATURA
TEMPERATURE RESISTANCE
TOMATE
TOMATOES
TRAITEMENT THERMIQUE
TRATAMIENTO TERMICO
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The kinetic parameters of heat-induced inactivation of tomato fruit pectic enzymes in a partially purified form have been calculated. Polygalacturonase I (PGI), polygalacturonase II (PGII) and pectinmethylesterase (PME) were separated by means of gel permeation chromatography and their thermoresistance in 50 mM citrate buffer, pH 4.0, 0,4 M. NaCl calculated. All the enzymes showed first-order kinetics of inactivation. PG II D values ranged from 2.14 min. at 64 deg. C to 0,24 Min. at 73 deg. C. Its z value was calculated to be 9.4 deg. C. PGI D values ranged from 15.9 min at 86 deg. C to 0.46 min at 95.4deg. C. Its z value was calculated to be 5.6 deg. C. PME D values ranged from 7.6 min at 66.4 deg. C to 0.20 min at 74.5 deg. C. Its z value was calculated to be 5 deg. C.
ISSN:1431-4630
1432-2331
DOI:10.1007/s002170050051