Optimization of structured diacylglycerols production containing ω-3 fatty acids via enzyme-catalysed glycerolysis of fish oil

The main objective of the present work was the production of structured diacylglycerols (DAG) by Novozym 435-catalysed glycerolysis of n-3 PUFA rich fish oil. Different enzyme loads (5, 7, 10, 13 and 15 %, with respect to the total weight of substrates), temperatures (40, 44, 50, 56 and 60 °C) and m...

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Bibliographic Details
Published in:European food research & technology 2013-03, Vol.236 (3), p.435-440
Main Authors: Miranda, Kelhy, Baeza-Jiménez, Ramiro, Noriega-Rodríguez, Juan A., García, Hugo S., Otero, Cristina
Format: Article
Language:English
Subjects:
Agriculture
Analytical Chemistry
Biotechnology
Chemistry
Chemistry and Materials Science
Food Science
Forestry
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Summary:The main objective of the present work was the production of structured diacylglycerols (DAG) by Novozym 435-catalysed glycerolysis of n-3 PUFA rich fish oil. Different enzyme loads (5, 7, 10, 13 and 15 %, with respect to the total weight of substrates), temperatures (40, 44, 50, 56 and 60 °C) and molar ratios of substrates (1, 1.4, 2, 2.6 and 3, oil/glycerol) were explored by the response surface methodology in order to optimize the reaction system. Temperature showed the highest significant effect ( P  
ISSN:1438-2377
1438-2385
DOI:10.1007/s00217-012-1889-2