Abscisic acid signal transduction in epidermal cells of Pisum sativum L. Argenteum: both dehydrin mRNA accumulation and stomatal responses require protein phosphorylation and dephosphorylation

The effects of a number of treatments, both in planta and in vitro, on the accumulation of mRNA encoding dehydrin, an abscisic acid (ABA)-inducible protein, were determined for guard cells and mesophyll cells prepared from leaves of the Argenteum mutant of Pisum sativum L. Guard cells and mesophyll...

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Bibliographic Details
Published in:Planta 1997, Vol.202 (1), p.85-92
Main Authors: Hey, S.J, Bacon, A, Burnett, E, Neill, S.J. (Bristol Univ. (United Kingdom). Dept. of Biological Sciences)
Format: Article
Language:English
Subjects:
ABA
ARN
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
BIOSYNTHESIS
Cell physiology
CELLS
CELLULE
CELULAS
Cyclosporin
Cyclosporins
Dehydrin
ENZIMAS
ENZYME
ENZYMES
Epidermal cells
EPIDERME
EPIDERMIS
ESTOMA
ESTRES
EXPRESION GENICA
EXPRESSION DES GENES
FEUILLE
FOSFORILACION
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
Guard cells
HOJAS
LEAVES
MESOFILO
MESOPHYLL
Mesophyll cells
MESOPHYLLE
Messenger RNA
Molecular and cellular biology
MUTANT
MUTANTES
MUTANTS
OSMOSE
OSMOSIS
Peas
Phorbolmyristatacetat
Phosphatases
PHOSPHORYLATION
PISUM SATIVUM
Plant physiology and development
PROTEIN KINASE
PROTEINA QUINASA
PROTEINAS
PROTEINE
PROTEINE KINASE
Proteinphosphatase
PROTEINS
RNA
Signal transduction
Signaltransduktion
STOMATA
STOMATE
STRESS
Water and solutes. Absorption, translocation and permeability
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Summary:The effects of a number of treatments, both in planta and in vitro, on the accumulation of mRNA encoding dehydrin, an abscisic acid (ABA)-inducible protein, were determined for guard cells and mesophyll cells prepared from leaves of the Argenteum mutant of Pisum sativum L. Guard cells and mesophyll cells treated for 10 d with ABA in planta accumulated dehydrin mRNA. However, after 1 or 3 d treatment, dehydrin mRNA was induced only in guard cells. Wilting induced dehydrin mRNA accumulation in leaves and epidermal cells. Induction of mRNA in epidermal cells was correlated with an increased ABA content after either ABA application or following wilting. Isolated mesophyll and guard cells responded to ABA in vitro by the induction of dehydrin mRNA. However, osmotic stress, imposed by incubation in mannitol, had no effect on ABA and dehydrin mRNA synthesis in mesophyll cells, and only a slight effect on guard cells. Both protein phosphorylation and dephosphorylation were shown to be required for ABA-induced dehydrin gene expression and stomatal movements. Inhibitors of protein kinases (K-252a) and protein phosphatases 1/2A (okadaic acid) and protein phosphatase 2B (cyclosporin A) all inhibited ABA-induced dehydrin mRNA accumulation. They also reduced the inhibitory effects of ABA on stomatal opening, as did the protein kinase activator phorbol myristate acetate (PMA). While K-252a, cyclosporin A and PMA also inhibited ABA-induced stomatal closure, okadaic acid enhanced the ABA effect, indicating the involvement of multiple protein phosphorylation/dephosphorylation steps in ABA signal transduction in guard cells.
ISSN:0032-0935
1432-2048
DOI:10.1007/s004250050106