Dormancy-associated gene expression in pea axillary buds

Pea (Pisum sativum L. cv. Alaska) axillary buds can be stimulated to cycle between dormant and growing states. Dormant buds synthesize unique proteins and are as metabolically active as growing buds. Two cDNAs. PsDRM1 and PsDRM2, were isolated from a dormant bud library. The deduced amino acid seque...

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Bibliographic Details
Published in:Planta 1998-06, Vol.205 (4), p.547-552
Main Authors: Stafstrom, J.P, Ripley, B.D, Devitt, M.L, Drake, B
Format: Article
Language:English
Subjects:
amino acid sequences
apical dominance
biomarkers
buds
complementary DNA
dormancy
dormancy breaking
genbank/af029242
genbank/af029243
gene expression
growth
messenger RNA
metabolism
molecular sequence data
nucleotide sequences
Pisum sativum
protein synthesis
roots
stems
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Summary:Pea (Pisum sativum L. cv. Alaska) axillary buds can be stimulated to cycle between dormant and growing states. Dormant buds synthesize unique proteins and are as metabolically active as growing buds. Two cDNAs. PsDRM1 and PsDRM2, were isolated from a dormant bud library. The deduced amino acid sequence of PsDRM1 (111 residues) is 75% identical to that of an auxin-repressed strawberry clone. PsDRM2 encodes a putative protein containing 129 residues, which includes 11 repeats of the sequence [G]-GGGY[H][N] (the bracketed residues may be absent). PsDRM2 is related to cold- and ABA-stimulated clones from alfalfa. Decapitating the terminal bud rapidly stimulates dormant axillary buds to begin growing. The abundance of PsDRM1 mRNA in axillary buds declines 20-fold within 6 h of decapitation; it quickly reaccumulates when buds become dormant again. The level of PsDRM2 mRNA is about three fold lower in growing buds than in dormant buds. Expression of PsDRM1 is enhanced in other non-growing organs (roots >>root apices; fully-elongated stems > elongating stems), and thus is an excellent "dormancy" marker. In contrast, PsDRM2 expression is not dormancy-associated in other organs.
ISSN:0032-0935
1432-2048
DOI:10.1007/s004250050354