Synthesis and localization of the mucin-associated TFF-peptides in the human uterus

TFF-peptides (formerly P-domain peptides, trefoil factors) are typical secretory products of mucin-producing cells and are thought to influence the rheological properties of mucous gels. Here, the localization of these peptides in the human uterus was investigated. An analysis of TFF-peptides mRNA b...

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Bibliographic Details
Published in:Cell and tissue research 2001-01, Vol.303 (1), p.109-115
Main Authors: Wiede, A, Hinz, M, Canzler, E, Franke, K, Quednow, C, Hoffmann, W
Format: Article
Language:English
Subjects:
Blotting, Western
Cervix Uteri - chemistry
Cervix Uteri - metabolism
Endometrium - chemistry
Endometrium - metabolism
Female
Fluorescent Antibody Technique
Gene Expression - physiology
Growth Substances - analysis
Growth Substances - genetics
Humans
Mucins - metabolism
Muscle Proteins
Neuropeptides
Oligonucleotide Probes
Peptides - analysis
Peptides - genetics
Pregnancy
Proteins - analysis
Proteins - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Trefoil Factor-2
Trefoil Factor-3
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Summary:TFF-peptides (formerly P-domain peptides, trefoil factors) are typical secretory products of mucin-producing cells and are thought to influence the rheological properties of mucous gels. Here, the localization of these peptides in the human uterus was investigated. An analysis of TFF-peptides mRNA by the polymerase chain reaction revealed TFF3 mainly in the endocervix and smaller amounts in the endometrium. TFF1 and TFF2 mRNA was detectable occasionally in the endocervix and very rarely in the endometrium. Western blot analysis detected only TFF3 in tissue extracts of the endocervix and as a constituent of human cervical mucus. Immunofluorescence localized TFF3 in the surface epithelium of the endocervix and in gland-like structures of the cervical epithelium.
ISSN:0302-766X
1432-0878
DOI:10.1007/s004410000297