The projections of 5-hydroxytryptamine-accumulating neurones in the myenteric plexus of the small intestine of the guinea-pig

Retrograde tracing, combined with immunohistochemistry, was used to study the projections of 5-hydroxytryptamine (5-HT)-accumulating neurones within the ileum of the guinea-pig, with confocal microscopy being used to characterise further their morphology. Two classes of neurones in the myenteric ple...

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Bibliographic Details
Published in:Cell and tissue research 1998-03, Vol.291 (3), p.375-384
Main Authors: Meedeniya, A C, Brookes, S J, Hennig, G W, Costa, M
Format: Article
Language:English
Subjects:
Animals
Axonal Transport
Carbocyanines
Dendrites - ultrastructure
Female
Fluorescent Dyes
Guinea Pigs
Immunohistochemistry
Male
Microscopy, Confocal
Myenteric Plexus - cytology
Myenteric Plexus - physiology
Neurons - cytology
Neurons - physiology
Serotonin - analysis
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Summary:Retrograde tracing, combined with immunohistochemistry, was used to study the projections of 5-hydroxytryptamine (5-HT)-accumulating neurones within the ileum of the guinea-pig, with confocal microscopy being used to characterise further their morphology. Two classes of neurones in the myenteric plexus, capable of taking up 5-HT or analogues, were distinguished. One class had Dogiel type I morphology with lamellar dendrites, was located on the edge or in the middle of ganglia and lacked immunoreactivity for somatostatin (SOM). The other class had smooth ovoid cell bodies with multiple filamentous dendrites and a single axon and represented a subset of the SOM-immunoreactive interneurones in the myenteric plexus. Varicosities immunoreactive for 5-HT alone, 5-HT/SOM or SOM alone were present in the myenteric ganglia. Both classes of 5-HT-accumulating neurones had long aboral projections within the myenteric plexus (up to 100 mm long) and to the submucous plexus and probably function as descending interneurones.
ISSN:0302-766X
1432-0878
DOI:10.1007/s004410051007