Urinary trypsin inhibitor ameliorates renal tissue oxygenation after ischemic reperfusion in rats

• Published in: Journal of anesthesia 2008-05, Vol.22 (2), p.149-154
• Main Authors: Taie, Satoshi, Ueki, Masaaki, Chujo, Kosuke, Asaga, Takehiko, Iwanaga, Yasuyuki, Ono, Junichiro, Maekawa, Nobuhiro
• Format: Article
• Language: English
• Subjects: Anesthesiology; Animals; Blood Gas Analysis; Blood Pressure - drug effects; Critical Care Medicine; Disease Models, Animal; Dose-Response Relationship, Drug; Emergency Medicine; Intensive; Kidney Cortex - drug effects; Kidney Medulla - drug effects; Male; Medicine; Medicine & Public Health; Original Article; Pain Medicine; Rats; Rats, Wistar; Reperfusion Injury - drug therapy; Treatment Outcome; Trypsin Inhibitors - administration & dosage; Trypsin Inhibitors - pharmacology
• ISSN: 0913-8668; 1438-8359
• DOI: 10.1007/s00540-007-0602-2

Purpose In order to determine the mechanism of the protective effect of a urinary trypsin inhibitor (UTI) on renal ischemic reperfusion injury, we measured the tissue oxygen partial pressure ( ) in both the renal cortex and medulla in rats, using electron paramagnetic resonance (EPR) oximetry. Methods We allocated the rats to three groups: normal saline (NS) group, a UTI 50 000 U·kg −1 (LD) group, and a UTI 150 000 U·kg −1 (HD) group, with the normal saline and UTI being administered 30 min before ischemia. Renal ischemia was achieved by inflating the balloon of a vascular occluder that had been placed around the abdominal aorta just above the bifurcation of the renal artery. Cortical and medullary were measured every 10 min during ischemia (30 min) and reperfusion (60 min) by EPR oximetry; also, systemic cardiopulmonary parameters were measured. Results The in the cortex and medulla decreased to less than 2 mmHg during ischemia in all groups. At 60 min after reperfusion, the values in the NS group were not fully restored, whereas those in the LD and HD groups were completely restored to the pre-ischemic values. There were no significant differences between the HD and LD groups. There were no differences between any groups in cardiopulmonary parameters. Conclusion Because UTI improved renal oxygenation after reperfusion without changing cardiopulmonary parameters, the pharmacological properties of UTI, such as its renal protection and anti-shock activity, may be explained in part, by this improvement in tissue oxygenation.