The features of adipose-derived stem cells in patients with inflammatory bowel diseases

• Published in: Surgery today (Tokyo, Japan) Japan), 2018-03, Vol.48 (3), p.352-358
• Main Authors: Mizushima, Tsunekazu, Fukata, Tadafumi, Takeyama, Hiroshi, Takahashi, Hidekazu, Haraguchi, Naotsugu, Nishimura, Junichi, Hata, Taishi, Matsuda, Chu, Yamamoto, Hirofumi, Doki, Yuichiro, Mori, Masaki
• Format: Article
• Language: English
• Subjects: Adipose Tissue - cytology; Adult; Aged; Animals; Cell Movement - drug effects; Cell Proliferation - drug effects; Cell Separation; Cell- and Tissue-Based Therapy - methods; Cells, Cultured; Female; Humans; Inflammation Mediators - adverse effects; Inflammatory Bowel Diseases - etiology; Inflammatory Bowel Diseases - pathology; Inflammatory Bowel Diseases - therapy; Male; Medicine; Medicine & Public Health; Mice; Middle Aged; Original Article; Stem Cells - cytology; Surgery; Surgical Oncology; Treatment Outcome; Tumor Necrosis Factor-alpha - adverse effects
• ISSN: 0941-1291; 1436-2813
• DOI: 10.1007/s00595-017-1591-x

Purpose The treatment outcomes for inflammatory bowel disease (IBD) have been improving, but the development of better therapies is needed. Stem cell therapy is promising, but little is known about the differences in adipose-derived stem cells (ADSCs) between IBD patients and healthy individuals. Methods ADSCs were isolated from subcutaneous adipose tissue (SAT) in IBD (Crohn’s disease, 3; ulcerative colitis, 2) and non-IBD (colorectal cancer, 5; breast cancer, 1) patients. We also analyzed the effects of tumor necrosis factor (TNF)-α on murine ADSCs. Results The numbers of stromal vascular fraction (SVF) cells per gram of SAT were 7.72 ± 3.03 × 10 5 in IBD and 8.51 ± 8.80 × 10 5 in non-IBD patients ( p  > 0.05). The proportions of ADSCs in SVF cells were 4.98 ± 2.61% in IBD and 1.02 ± 0.67% in non-IBD patients. The numbers of ADSCs per gram of SAT were 4.16 ± 2.96 × 10 4 in IBD and 0.88 ± 1.04 × 10 4 in non-IBD patients. The proportions and numbers of ADSCs were significantly higher in IBD patients than in non-IBD patients. TNF-α significantly facilitated the proliferation and motility of murine ADSCs. Conclusion These results showed the potential advantage of freshly isolated autologous ADSCs in IBD patients.