Sandwich-type amperometric immunosensor for human immunoglobulin G using antibody-adsorbed Au/SiO₂ nanoparticles

A novel sandwich-type electrochemical immunosensor for human immunoglobulin G (hIgG) was developed using Au/SiO₂ nanoparticles (NPs) with adsorbed horseradish peroxidase-anti-hIgG as the secondary antibody layer. The signal readout is based on the amperometric response to the catalytic reduction of...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2010-03, Vol.168 (3-4), p.245-251
Main Authors: Wang, Lingling, Jia, Xueen, Zhou, Yaping, Xie, Qingji, Yao, Shouzhuo
Format: Article
Language:English
Subjects:
Analytical Chemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Electrochemical methods
Exact sciences and technology
General, instrumentation
Microengineering
Nanochemistry
Nanotechnology
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Summary:A novel sandwich-type electrochemical immunosensor for human immunoglobulin G (hIgG) was developed using Au/SiO₂ nanoparticles (NPs) with adsorbed horseradish peroxidase-anti-hIgG as the secondary antibody layer. The signal readout is based on the amperometric response to the catalytic reduction of hydrogen peroxide at an AuNPs-polythionine modified glassy carbon electrode. Under optimized conditions, the linear range is from 0.1 to 200 ng·mL⁻¹, with a detection limit of 0.035 ng·mL⁻¹ (at an S/N of 3). The immunosensor exhibited a performance that is better than that based on Au/SiO₂NPs-excluded secondary antibody.
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-009-0281-9