Sandwich-type amperometric immunosensor for human immunoglobulin G using antibody-adsorbed Au/SiO₂ nanoparticles

A novel sandwich-type electrochemical immunosensor for human immunoglobulin G (hIgG) was developed using Au/SiO₂ nanoparticles (NPs) with adsorbed horseradish peroxidase-anti-hIgG as the secondary antibody layer. The signal readout is based on the amperometric response to the catalytic reduction of...

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Published in:Mikrochimica acta (1966) 2010-03, Vol.168 (3-4), p.245-251
Main Authors: Wang, Lingling, Jia, Xueen, Zhou, Yaping, Xie, Qingji, Yao, Shouzhuo
Format: Article
Language:English
Subjects:
Analytical Chemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Electrochemical methods
Exact sciences and technology
General, instrumentation
Microengineering
Nanochemistry
Nanotechnology
Original Paper
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cited_by cdi_FETCH-LOGICAL-c342t-2b353e6dfa7d013d934d442d036d08113ac202e27070ca895a389c4ed0c6283e3
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container_issue 3-4
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container_title Mikrochimica acta (1966)
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creator Wang, Lingling
Jia, Xueen
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Yao, Shouzhuo
description A novel sandwich-type electrochemical immunosensor for human immunoglobulin G (hIgG) was developed using Au/SiO₂ nanoparticles (NPs) with adsorbed horseradish peroxidase-anti-hIgG as the secondary antibody layer. The signal readout is based on the amperometric response to the catalytic reduction of hydrogen peroxide at an AuNPs-polythionine modified glassy carbon electrode. Under optimized conditions, the linear range is from 0.1 to 200 ng·mL⁻¹, with a detection limit of 0.035 ng·mL⁻¹ (at an S/N of 3). The immunosensor exhibited a performance that is better than that based on Au/SiO₂NPs-excluded secondary antibody.
doi_str_mv 10.1007/s00604-009-0281-9
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subjects Analytical Chemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Electrochemical methods
Exact sciences and technology
General, instrumentation
Microengineering
Nanochemistry
Nanotechnology
Original Paper
title Sandwich-type amperometric immunosensor for human immunoglobulin G using antibody-adsorbed Au/SiO₂ nanoparticles
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