A novel and simple biosensor based on poly(indoleacetic acid) film and its application for simultaneous electrochemical determination of dopamine and epinephrine in the presence of ascorbic acid

• Published in: Journal of solid state electrochemistry 2012-06, Vol.16 (6), p.2203-2210
• Main Authors: Zhou, Yuanzhen, He, Maofang, Huang, Cancan, Dong, Sheying, Zheng, Jianbin
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Condensed Matter Physics; Electrochemistry; Energy Storage; Original Paper; Physical Chemistry
• ISSN: 1432-8488; 1433-0768
• DOI: 10.1007/s10008-012-1646-2

A novel and simple biosensor based on poly(indoleacetic acid) film-modified electrode (PIAA/CPE) was fabricated by electrochemical polymerization of indoleacetic acid on a carbon paste electrode (CPE) through cyclic voltammetry. The resulting electrode was characterized by scanning electron microscopy, and the electrochemical behaviors of dopamine (DA) and epinephrine (EP) at the electrode were studied. It was illustrated that PIAA/CPE had excellent electrochemical catalytic activities toward DA and EP. The anodic peak currents ( I pa ) were dramatically enhanced by about seven-fold for DA and ten times for EP at PIAA/CPE. Thus, the determinations of DA and EP were carried out using PIAA/CPE successfully. The linear responses were obtained in the range of 3.0 × 10 −7 ∼7.0 × 10 −4 and 1.0 × 10 −6 ∼8.0 × 10 −4  mol L −1 with the detection limits (3 σ ) of 1 × 10 −7 and 4 × 10 −7  mol L −1 corresponding with DA and EP, respectively. Moreover, the cathodic peaks of DA and EP were well-separated with a potential difference about 325 mV in pH 5.3 phosphate-buffered saline, so simultaneous determination of DA and EP was carried out in this paper. Additionally, the interference studies showed that the PIAA/CPE exhibited excellent selectivity in the presence of ascorbic acid (AA). With good selectivity and sensitivity, the present method has been successfully applied to the determination of DA and EP in pharmaceutical samples.