Direct electrochemistry and electrocatalysis of hemoglobin on a gold ion implantation-modified indium tin oxide electrode

• Published in: Journal of solid state electrochemistry 2013-03, Vol.17 (3), p.655-660
• Main Authors: Jia, Mingzhe, Liang, Fenfen, Jiao, Jiao, Li, Shuoqi, Hu, Jingbo
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Condensed Matter Physics; Electrochemistry; Energy Storage; Original Paper; Physical Chemistry
• ISSN: 1432-8488; 1433-0768
• DOI: 10.1007/s10008-012-1906-1

In this paper, a gold nanoparticle-modified indium tin oxide electrode (Au/ITO) was prepared without the use of any cross-linker or stabilizer reagent. The prepared Au/ITO was used as a new platform to achieve the direct electron transfer between Hb and the modified electrode. The proposed electrode exhibited a pair of well-defined redox peaks with a formal potential of −0.073 V (vs. Ag/AgCl). The immobilized Hb showed excellent electrocatalytic activity toward H 2 O 2 and the electrocatalytic current values were linear with the increasing concentration of H 2 O 2 ranging from 1.0 × 10 −6  M to 7.0 × 10 −4  M. The detection limit was 2.0 × 10 −7  M (S/N = 3) and the Michaelis–Menten constant was calculated to be 0.2 mM. The proposed electrode also showed high selectivity, long-term stability, and good reproducibility.