SPE of Tanshinones from Salvia miltiorrhiza Bunge by using Imprinted Functionalized Ionic Liquid-Modified Silica

• Published in: Chromatographia 2011, Vol.73 (1-2), p.25-31
• Main Authors: Tian, Minglei, Row, Kyung Ho
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Biological and medical sciences; Chemistry; Chemistry and Materials Science; Chromatography; Column liquid chromatography; Food industries; Fundamental and applied biological sciences. Psychology; General pharmacology; Laboratory Medicine; Medical sciences; Molecular imprinted ionic liquid-modified silica; Non alcoholic beverage industries and mineral waters; Original; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Pharmacy; Proteomics; Salvia miltiorrhiza Bunge; solid phase extraction; Tanshinones
• ISSN: 0009-5893; 1612-1112
• DOI: 10.1007/s10337-010-1836-y

Ionic liquid-modified silica, with functional groups based on imidazole as the cation, was obtained. A molecular imprinting technique was introduced to form the order of functional groups. The selectivity of the obtained ionic liquid-modified silica was successfully used as a special imprinted sorbent in the solid-phase extraction to isolate cryptotanshinone, tanshinone I and tanshinone IIA from Salvia miltiorrhiza Bunge. Several washing and elution solvents with different polarities were evaluated. The ionic liquid-modified silica as the sorbent exhibited a higher selectivity than blank ionic liquid-modified silica, traditional silica and C₁₈ cartridges. A quantitative analysis was conducted by liquid chromatography with a C₁₈ column and methanol/water (75:25, v/v, containing 0.5% acetic acid) as the mobile phase. A good linearity was obtained from 0.5 × 10⁻⁴ to 0.1 mg mL⁻¹ (r ² > 0.99) with relative standard deviations that were less than 4.6%.