Simultaneous Determination of Strychnine, Brucine, Strychnine N-Oxide and Brucine N-Oxide in Crude and Fermented Nux Vomica by CZE with Ephedrine Hydrochloride as an Internal Standard

A rapid capillary zone electrophoresis (CZE) method with ephedrine hydrochloride as an internal standard was developed and validated for separating and quantifying the alkaloids strychnine ( 1 ), brucine ( 2 ), strychnine N -oxide ( 3 ), and brucine N -oxide ( 4 ) in the seed of Strychnos nux - vomi...

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Bibliographic Details
Published in:Chromatographia 2011-06, Vol.73 (11-12), p.1223-1228
Main Authors: Yang, Guangming, Pan, Yang, Liu, Liangjing, Jiang, Yaping
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biological and medical sciences
Chemistry
Chemistry and Materials Science
Chromatography
Full Short Communication
General pharmacology
Laboratory Medicine
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Pharmacy
Proteomics
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Summary:A rapid capillary zone electrophoresis (CZE) method with ephedrine hydrochloride as an internal standard was developed and validated for separating and quantifying the alkaloids strychnine ( 1 ), brucine ( 2 ), strychnine N -oxide ( 3 ), and brucine N -oxide ( 4 ) in the seed of Strychnos nux - vomica L. (crude nux vomica) and its fermented products by fungus Trametes cinnabarina (Jacq.: Fr.) Fr. (fermented nux vomica). The contents of alkaloids ( 1 and 2) in crude nux vomica were higher than those in fermented drugs, whereas alkaloids ( 3 and 4) were only detected in fermented ones. The method developed is sensitive, specific and accurate, as well as effective and easy, which can be used to assess the qualities of crude and fermented nux vomica.
ISSN:0009-5893
1612-1112
DOI:10.1007/s10337-011-1912-y