MEKC Determination of Nilutamide in Human Serum Using Sweeping in High Salt Sample Matrix

The determination of the antiandrogen drug nilutamide in human serum by MEKC using flutamide as an internal standard is described. Several parameters influencing the separation, such as the running electrolyte composition concerning the micelle concentration and pH, are discussed. MEKC separation wa...

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Bibliographic Details
Published in:Chromatographia 2011-07, Vol.74 (1-2), p.151-155
Main Authors: Znaleziona, Joanna, Maier, Vítězslav, Petr, Jan, Chrastina, Jan, Ševčík, Juraj
Format: Article
Language:English
Subjects:
Analytical Chemistry
Antineoplastic agents
Biological and medical sciences
Chemistry
Chemistry and Materials Science
Chromatography
Full Short Communication
General aspects
Laboratory Medicine
Medical sciences
Pharmacology. Drug treatments
Pharmacy
Proteomics
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Summary:The determination of the antiandrogen drug nilutamide in human serum by MEKC using flutamide as an internal standard is described. Several parameters influencing the separation, such as the running electrolyte composition concerning the micelle concentration and pH, are discussed. MEKC separation was achieved within 7 min using 50 mM sodium borate pH 9.0 with the addition of 50 mM sodium dodecylsulfate at +20 kV. The proposed method was applied to determination of nilutamide in spiked human serum samples after protein precipitation with acetonitrile. The increasing of sensitivity for determination of nilutamide in serum was done by sweeping in a high salt concentration sample matrix when the injection of a larger volume of sample diluted in 150 mM NaCl was applied. The limit of detection after the preconcentration step for nilutamide was 26 μg L −1 .
ISSN:0009-5893
1612-1112
DOI:10.1007/s10337-011-2019-1