MEKC Determination of Nilutamide in Human Serum Using Sweeping in High Salt Sample Matrix

The determination of the antiandrogen drug nilutamide in human serum by MEKC using flutamide as an internal standard is described. Several parameters influencing the separation, such as the running electrolyte composition concerning the micelle concentration and pH, are discussed. MEKC separation wa...

Full description

Saved in:
Bibliographic Details
Published in:Chromatographia 2011-07, Vol.74 (1-2), p.151-155
Main Authors: Znaleziona, Joanna, Maier, Vítězslav, Petr, Jan, Chrastina, Jan, Ševčík, Juraj
Format: Article
Language:English
Subjects:
Analytical Chemistry
Antineoplastic agents
Biological and medical sciences
Chemistry
Chemistry and Materials Science
Chromatography
Full Short Communication
General aspects
Laboratory Medicine
Medical sciences
Pharmacology. Drug treatments
Pharmacy
Proteomics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c458t-4de21a4295a89ee43eaf120632ba5afc89892ffef60347eab2a3c7f0e58dee2f3
cites cdi_FETCH-LOGICAL-c458t-4de21a4295a89ee43eaf120632ba5afc89892ffef60347eab2a3c7f0e58dee2f3
container_end_page 155
container_issue 1-2
container_start_page 151
container_title Chromatographia
container_volume 74
creator Znaleziona, Joanna
Maier, Vítězslav
Petr, Jan
Chrastina, Jan
Ševčík, Juraj
description The determination of the antiandrogen drug nilutamide in human serum by MEKC using flutamide as an internal standard is described. Several parameters influencing the separation, such as the running electrolyte composition concerning the micelle concentration and pH, are discussed. MEKC separation was achieved within 7 min using 50 mM sodium borate pH 9.0 with the addition of 50 mM sodium dodecylsulfate at +20 kV. The proposed method was applied to determination of nilutamide in spiked human serum samples after protein precipitation with acetonitrile. The increasing of sensitivity for determination of nilutamide in serum was done by sweeping in a high salt concentration sample matrix when the injection of a larger volume of sample diluted in 150 mM NaCl was applied. The limit of detection after the preconcentration step for nilutamide was 26 μg L −1 .
doi_str_mv 10.1007/s10337-011-2019-1
format article
fullrecord <record><control><sourceid>pascalfrancis_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1007_s10337_011_2019_1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>24292809</sourcerecordid><originalsourceid>FETCH-LOGICAL-c458t-4de21a4295a89ee43eaf120632ba5afc89892ffef60347eab2a3c7f0e58dee2f3</originalsourceid><addsrcrecordid>eNp9ULlOAzEQtRBIhMAH0LmhXPDYe7lEIRAEgSKkoFpNNuPgaC_ZuwL-Hq-CKGnm0Ds08xi7BHENQmQ3HoRSWSQAIilAR3DEJpCCjABAHrOJEEJHSa7VKTvzfh9WqdN0wt6X86cZv6OeXG0b7G3b8NbwF1sNPdZ2S9w2fDHU2PAVuaHma2-bHV99EnXjMKJ298FXWPWh1F1FfIm9s1_n7MRg5enit0_Z-n7-NltEz68Pj7Pb56iMk7yP4i1JwFjqBHNNFCtCA1KkSm4wQVPmOtfSGDKpUHFGuJGoyswISvItkTRqyuDgW7rWe0em6Jyt0X0XIIoxm-KQTRGyKcZsCgiaq4OmQ19iZRw2pfV_QhnOkbnQgScPPB-gZkeu2LeDa8I7_5j_AFSLdAY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>MEKC Determination of Nilutamide in Human Serum Using Sweeping in High Salt Sample Matrix</title><source>Springer Link</source><creator>Znaleziona, Joanna ; Maier, Vítězslav ; Petr, Jan ; Chrastina, Jan ; Ševčík, Juraj</creator><creatorcontrib>Znaleziona, Joanna ; Maier, Vítězslav ; Petr, Jan ; Chrastina, Jan ; Ševčík, Juraj</creatorcontrib><description>The determination of the antiandrogen drug nilutamide in human serum by MEKC using flutamide as an internal standard is described. Several parameters influencing the separation, such as the running electrolyte composition concerning the micelle concentration and pH, are discussed. MEKC separation was achieved within 7 min using 50 mM sodium borate pH 9.0 with the addition of 50 mM sodium dodecylsulfate at +20 kV. The proposed method was applied to determination of nilutamide in spiked human serum samples after protein precipitation with acetonitrile. The increasing of sensitivity for determination of nilutamide in serum was done by sweeping in a high salt concentration sample matrix when the injection of a larger volume of sample diluted in 150 mM NaCl was applied. The limit of detection after the preconcentration step for nilutamide was 26 μg L −1 .</description><identifier>ISSN: 0009-5893</identifier><identifier>EISSN: 1612-1112</identifier><identifier>DOI: 10.1007/s10337-011-2019-1</identifier><identifier>CODEN: CHRGB7</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Analytical Chemistry ; Antineoplastic agents ; Biological and medical sciences ; Chemistry ; Chemistry and Materials Science ; Chromatography ; Full Short Communication ; General aspects ; Laboratory Medicine ; Medical sciences ; Pharmacology. Drug treatments ; Pharmacy ; Proteomics</subject><ispartof>Chromatographia, 2011-07, Vol.74 (1-2), p.151-155</ispartof><rights>Springer-Verlag 2011</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-4de21a4295a89ee43eaf120632ba5afc89892ffef60347eab2a3c7f0e58dee2f3</citedby><cites>FETCH-LOGICAL-c458t-4de21a4295a89ee43eaf120632ba5afc89892ffef60347eab2a3c7f0e58dee2f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=24292809$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Znaleziona, Joanna</creatorcontrib><creatorcontrib>Maier, Vítězslav</creatorcontrib><creatorcontrib>Petr, Jan</creatorcontrib><creatorcontrib>Chrastina, Jan</creatorcontrib><creatorcontrib>Ševčík, Juraj</creatorcontrib><title>MEKC Determination of Nilutamide in Human Serum Using Sweeping in High Salt Sample Matrix</title><title>Chromatographia</title><addtitle>Chromatographia</addtitle><description>The determination of the antiandrogen drug nilutamide in human serum by MEKC using flutamide as an internal standard is described. Several parameters influencing the separation, such as the running electrolyte composition concerning the micelle concentration and pH, are discussed. MEKC separation was achieved within 7 min using 50 mM sodium borate pH 9.0 with the addition of 50 mM sodium dodecylsulfate at +20 kV. The proposed method was applied to determination of nilutamide in spiked human serum samples after protein precipitation with acetonitrile. The increasing of sensitivity for determination of nilutamide in serum was done by sweeping in a high salt concentration sample matrix when the injection of a larger volume of sample diluted in 150 mM NaCl was applied. The limit of detection after the preconcentration step for nilutamide was 26 μg L −1 .</description><subject>Analytical Chemistry</subject><subject>Antineoplastic agents</subject><subject>Biological and medical sciences</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography</subject><subject>Full Short Communication</subject><subject>General aspects</subject><subject>Laboratory Medicine</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Pharmacy</subject><subject>Proteomics</subject><issn>0009-5893</issn><issn>1612-1112</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9ULlOAzEQtRBIhMAH0LmhXPDYe7lEIRAEgSKkoFpNNuPgaC_ZuwL-Hq-CKGnm0Ds08xi7BHENQmQ3HoRSWSQAIilAR3DEJpCCjABAHrOJEEJHSa7VKTvzfh9WqdN0wt6X86cZv6OeXG0b7G3b8NbwF1sNPdZ2S9w2fDHU2PAVuaHma2-bHV99EnXjMKJ298FXWPWh1F1FfIm9s1_n7MRg5enit0_Z-n7-NltEz68Pj7Pb56iMk7yP4i1JwFjqBHNNFCtCA1KkSm4wQVPmOtfSGDKpUHFGuJGoyswISvItkTRqyuDgW7rWe0em6Jyt0X0XIIoxm-KQTRGyKcZsCgiaq4OmQ19iZRw2pfV_QhnOkbnQgScPPB-gZkeu2LeDa8I7_5j_AFSLdAY</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Znaleziona, Joanna</creator><creator>Maier, Vítězslav</creator><creator>Petr, Jan</creator><creator>Chrastina, Jan</creator><creator>Ševčík, Juraj</creator><general>Springer-Verlag</general><general>Springer</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20110701</creationdate><title>MEKC Determination of Nilutamide in Human Serum Using Sweeping in High Salt Sample Matrix</title><author>Znaleziona, Joanna ; Maier, Vítězslav ; Petr, Jan ; Chrastina, Jan ; Ševčík, Juraj</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-4de21a4295a89ee43eaf120632ba5afc89892ffef60347eab2a3c7f0e58dee2f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analytical Chemistry</topic><topic>Antineoplastic agents</topic><topic>Biological and medical sciences</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatography</topic><topic>Full Short Communication</topic><topic>General aspects</topic><topic>Laboratory Medicine</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Pharmacy</topic><topic>Proteomics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Znaleziona, Joanna</creatorcontrib><creatorcontrib>Maier, Vítězslav</creatorcontrib><creatorcontrib>Petr, Jan</creatorcontrib><creatorcontrib>Chrastina, Jan</creatorcontrib><creatorcontrib>Ševčík, Juraj</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Chromatographia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Znaleziona, Joanna</au><au>Maier, Vítězslav</au><au>Petr, Jan</au><au>Chrastina, Jan</au><au>Ševčík, Juraj</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MEKC Determination of Nilutamide in Human Serum Using Sweeping in High Salt Sample Matrix</atitle><jtitle>Chromatographia</jtitle><stitle>Chromatographia</stitle><date>2011-07-01</date><risdate>2011</risdate><volume>74</volume><issue>1-2</issue><spage>151</spage><epage>155</epage><pages>151-155</pages><issn>0009-5893</issn><eissn>1612-1112</eissn><coden>CHRGB7</coden><abstract>The determination of the antiandrogen drug nilutamide in human serum by MEKC using flutamide as an internal standard is described. Several parameters influencing the separation, such as the running electrolyte composition concerning the micelle concentration and pH, are discussed. MEKC separation was achieved within 7 min using 50 mM sodium borate pH 9.0 with the addition of 50 mM sodium dodecylsulfate at +20 kV. The proposed method was applied to determination of nilutamide in spiked human serum samples after protein precipitation with acetonitrile. The increasing of sensitivity for determination of nilutamide in serum was done by sweeping in a high salt concentration sample matrix when the injection of a larger volume of sample diluted in 150 mM NaCl was applied. The limit of detection after the preconcentration step for nilutamide was 26 μg L −1 .</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><doi>10.1007/s10337-011-2019-1</doi><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0009-5893
ispartof Chromatographia, 2011-07, Vol.74 (1-2), p.151-155
issn 0009-5893
1612-1112
language eng
recordid cdi_crossref_primary_10_1007_s10337_011_2019_1
source Springer Link
subjects Analytical Chemistry
Antineoplastic agents
Biological and medical sciences
Chemistry
Chemistry and Materials Science
Chromatography
Full Short Communication
General aspects
Laboratory Medicine
Medical sciences
Pharmacology. Drug treatments
Pharmacy
Proteomics
title MEKC Determination of Nilutamide in Human Serum Using Sweeping in High Salt Sample Matrix
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T08%3A59%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pascalfrancis_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=MEKC%20Determination%20of%20Nilutamide%20in%20Human%20Serum%20Using%20Sweeping%20in%20High%20Salt%20Sample%20Matrix&rft.jtitle=Chromatographia&rft.au=Znaleziona,%20Joanna&rft.date=2011-07-01&rft.volume=74&rft.issue=1-2&rft.spage=151&rft.epage=155&rft.pages=151-155&rft.issn=0009-5893&rft.eissn=1612-1112&rft.coden=CHRGB7&rft_id=info:doi/10.1007/s10337-011-2019-1&rft_dat=%3Cpascalfrancis_cross%3E24292809%3C/pascalfrancis_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c458t-4de21a4295a89ee43eaf120632ba5afc89892ffef60347eab2a3c7f0e58dee2f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true