GC–MS Determination of PAHs in Fish Samples Following Salting-out-Assisted Solvent Extraction-Gel Permeation Chromatography

A fast and effective sample cleanup procedure for the quantification of polycyclic aromatic hydrocarbons (PAHs) in fish samples is presented. The procedure involved extraction of fish samples using acetonitrile and cleanup by an automated gel-permeation chromatography (GPC) following liquid–liquid p...

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Bibliographic Details
Published in:Chromatographia 2011-09, Vol.74 (5-6), p.477-482
Main Authors: Hassan, Jalal, Farahani, Abolfazl
Format: Article
Language:English
Subjects:
Agnatha. Pisces
Analytical Chemistry
Animal, plant and microbial ecology
Applied ecology
Biological and medical sciences
Chemistry
Chemistry and Materials Science
Chromatography
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on vertebrates
Full Short Communication
Fundamental and applied biological sciences. Psychology
Laboratory Medicine
Pharmacy
Proteomics
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Summary:A fast and effective sample cleanup procedure for the quantification of polycyclic aromatic hydrocarbons (PAHs) in fish samples is presented. The procedure involved extraction of fish samples using acetonitrile and cleanup by an automated gel-permeation chromatography (GPC) following liquid–liquid partition into n -hexane. The extracted samples were analyzed by gas chromatography-mass spectrometry (GC–MS). Electron ionization was employed in a single analysis for the determination of PAHs in the selected ion monitoring mode. Three different solvents were studied for the extraction step: acetonitrile/ n -hexane, methanol/ n -hexane and acetone/ n -hexane. The best solvent was found to be acetonitrile/ n -hexane. The cleanup technique resulted in a good separation of analytes from co-extractive matrix compounds.
ISSN:0009-5893
1612-1112
DOI:10.1007/s10337-011-2093-4