Analysis of Neutral Sugars of Asparagus officinalis Linn. Polysaccharide by CZE with Amperometric Detection

• Published in: Chromatographia 2012-03, Vol.75 (5-6), p.297-304
• Main Authors: Yang, Zhiyong, Wang, Huan, Zhang, Wen, Wang, Qingjiang, He, Pingang, Fang, Yuzhi
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Biological and medical sciences; Chemistry; Chemistry and Materials Science; Chromatography; General pharmacology; Laboratory Medicine; Medical sciences; Original; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Pharmacy; Proteomics
• ISSN: 0009-5893; 1612-1112
• DOI: 10.1007/s10337-012-2197-5

Analysis of the neutral sugars of Asparagus officinalis Linn. polysaccharide by different methods has yielded inconsistent results. In the work discussed in this paper, capillary zone electrophoresis with amperometric detection (CZE–AD) was used for analysis of neutral sugars in A. officinalis Linn. polysaccharide. The configuration of the wall-jet and the diameter of the copper disk electrode were investigated to achieve optimized detection sensitivity. The separation electrolyte, separation voltage, and injection time were studied for their effects on CZE separation. Under the optimum CZE–AD conditions, seven monosaccharides were separated to baseline by using 120 m M NaOH as separation electrolyte. Linear response was excellent and repeatability was satisfactory. It was found that Asparagus polysaccharide was composed of fucose, galactose, glucose, rhamnose, arabinose, fructose, and xylose at a mole ratio of 0.2:16.2:5.0:1.0:15.5:0.6:18.8. Compared with other methods, analysis of the composition of Asparagus polysaccharide by CZE–AD had the merits of rapidness, accuracy, and lower sampling volume.