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Quantitation by GC–MS of Methylglyoxal as a Marker in Anxiety-Related Studies

Methylglyoxal (MG) is present in the human system. In the cell, MG is degraded by glyoxalase 1 (Glo 1), an enzyme differentially expressed in several mouse models of anxiety-related behavior. It has also been associated with the formation of advanced glycation and lipoxidation end products, contribu...

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Published in:Chromatographia 2013-05, Vol.76 (9-10), p.571-576
Main Authors: Chen, Bud-Gen, Lin, Chia Han, Chen, Chinpiao, Hambsch, Boris, Chern, Chi-Liang
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Language:English
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cited_by cdi_FETCH-LOGICAL-c288t-368e1cf82c2a9b0ed6e8c98cdef1dc0762d4219447df4362f40dec5e31b820183
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creator Chen, Bud-Gen
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description Methylglyoxal (MG) is present in the human system. In the cell, MG is degraded by glyoxalase 1 (Glo 1), an enzyme differentially expressed in several mouse models of anxiety-related behavior. It has also been associated with the formation of advanced glycation and lipoxidation end products, contributing to diabetes-related complications. Approaches developed for accurate quantitation of MG in this study include the synthesis of a deuterated MG analog to serve as an internal standard; skillful application of the standard addition protocol (with “reiteration”) to quantitate MG in “blank” samples, allowing the preparation of calibration standards using the same test specimen matrix system; and various pretreatment methods, including extraction and derivatization, to prepare complex brain tissue specimens for gas chromatography–mass spectrometry analysis. Using our method, brain MG levels in high- and low-anxiety-related behavior (HAB and LAB) mice were determined as 5.11 ± 0.89 and 13.05 ± 0.89 μg g −1 , respectively. These data supported the conclusion that MG level in brain is negatively correlated with anxiety in mouse models of anxiety-related studies, contributing to better understanding of the molecular mechanisms underlying reported observations.
doi_str_mv 10.1007/s10337-013-2397-7
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subjects Analytical Chemistry
Chemistry
Chemistry and Materials Science
Chromatography
Laboratory Medicine
Pharmacy
Proteomics
Short Communication
title Quantitation by GC–MS of Methylglyoxal as a Marker in Anxiety-Related Studies
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