Enhancement of reproductive performances of Gangetic leaffish, Nandus nandus through up regulation of serum Ca2+ concentration, improved morphological alteration of liver and ovary with dietary polyunsaturated fatty acids

• Published in: Fish physiology and biochemistry 2013-08, Vol.39 (4), p.779-791
• Main Authors: Reza, A. H. M. M., Rakhi, S. F., Hossen, M. S., Takahashi, K., Hossain, Z.
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Animal Feed - analysis; Animal Physiology; Animals; Biomedical and Life Sciences; Calcium - blood; Decapodiformes - chemistry; Endangered Species; Fatty Acids, Omega-3 - analysis; Fatty Acids, Omega-3 - pharmacology; Female; Freshwater & Marine Ecology; Histology; Life Sciences; Liver - anatomy & histology; Liver - drug effects; Male; Morphology; Ovary - anatomy & histology; Ovary - drug effects; Ovary - growth & development; Perciformes - blood; Perciformes - growth & development; Phospholipids - chemistry; Reproduction - drug effects; Spermatozoa - drug effects; Zoology
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-012-9740-z

Incorporation of polyunsaturated fatty acids (PUFAs) as biofunctional compounds with feed is an effective way for gonadal maturation without any hazardous effects on animal health, and thus it is possible to save the vulnerable species from the danger of extinction. In the present study sperm quality, level of Ca 2+ concentration in serum, histological structure of the liver and developmental stages of ovary of an endangered fish species, Nandus nandus were investigated for the confirmation of the positive effects of PUFAs in reproduction and gonadal maturation. Fishes were collected from Brahmaputra River, Mymensingh, Bangladesh. Treated group was fed 1 % squid extracted phospholipid supplemented diet that was mixed with silver carp fish muscle where as controlled group was fed the same except phospholipid. For histology of liver and gonads, samples were dehydrated, cleaned and infiltrated, embedded in paraffin wax and sectioned. After that, the samples were stained with hematoxylin and eosin. The photomicrographs of the stained samples were taken by using light microscope. In comparison with the control group, treated group exhibited higher gonadal maturation which resulted in spontaneous spawning. Treated female demonstrated advanced gonadal developmental stages in comparison with the controlled female during different months. During spawning season, lipid granules and normal morphological alteration were observed in case of treated fish liver, whereas less lipid granules with more histological alteration of liver were observed in control group. Serum Ca 2+ concentration in treated female was found significantly higher ( P