Loading…
Immunomodulatory and therapeutic role of Cinnamomum verum extracts in collagen-induced arthritic BALB/c mice
Ethnopharmacological relevance Cinnamomum verum (CV), also known as ‘Dalchini’, is the dry bark of the Cinnamomum verum (L.) plant, and has been used as a traditional Pakistani medicine to alleviate pain and inflammation in patients suffering from arthritic rheumatism. It contains alkaloids, triterp...
Saved in:
| Published in: | Inflammopharmacology 2018-02, Vol.26 (1), p.157-170 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c344t-215249384023c1ca69f22fa99987999907e38cd68ba5cbe16cdd3ce2a2f4806a3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c344t-215249384023c1ca69f22fa99987999907e38cd68ba5cbe16cdd3ce2a2f4806a3 |
| container_end_page | 170 |
| container_issue | 1 |
| container_start_page | 157 |
| container_title | Inflammopharmacology |
| container_volume | 26 |
| creator | Qadir, Mirza Muhammad Fahd Bhatti, Attya Ashraf, Muhammad Usman Sandhu, Mansur Abdullah Anjum, Sidrah John, Peter |
| description | Ethnopharmacological relevance
Cinnamomum verum
(CV), also known as ‘Dalchini’, is the dry bark of the
Cinnamomum verum
(L.) plant, and has been used as a traditional Pakistani medicine to alleviate pain and inflammation in patients suffering from arthritic rheumatism. It contains alkaloids, triterpenes, Cinnamaldehyde and other volatile oils. The aim of the present study was to investigate the underlying biological effect of ethyl alcohol (EtOH) and methyl alcohol (MeOH) extracts from CV on collagen type-II induced arthritic (CIA) mice.
Materials and methods
Gas chromatography mass spectrophotometry was used to perform fingerprinting identification of the EtOH and MeOH extracts. CIA mice model was established by subdermal injections of type-II bovine collagen (CII) on the 1st, 8th and 14th day of the experiment. Ethyl alcohol extract and methyl alcohol extract (1 mg/KgBW, 2 mg/KgBW and 4 mg/KgBW), was orally administered from the 15th day onwards for 2 weeks. Progression of oedema and joint inflammation was measured in the paws using a digital Vernier calliper every 3 days from day 1 till the end of the experiment. The oxidative scavenging ability of cinnamaldehyde was evaluated using a DPPH assay. Similarly, the nitrogen free radical (NOS) production of isolated lymphocytes was evaluated using Greiss’s method. The spleen index was calculated and knee joint changes were observed by histopathological sectioning. Western blot analysis was performed on peripheral blood derived serum for CII, CAPN1, TNFα and NFATc3.
Results
Extracts were shown to be enriched in trans-cinnamaldehyde and its analogues. Extracts showed good ameliorative effects (
p
|
| doi_str_mv | 10.1007/s10787-017-0349-9 |
| format | article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1007_s10787_017_0349_9</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>28429097</sourcerecordid><originalsourceid>FETCH-LOGICAL-c344t-215249384023c1ca69f22fa99987999907e38cd68ba5cbe16cdd3ce2a2f4806a3</originalsourceid><addsrcrecordid>eNp9kM1OAyEUhYnR2Fp9ADeGF8ACwzCwbBt_mjRxo2tCGaadZoAGZox9e2lGXbq45y7uOSe5HwD3BD8SjKt5IrgSFcIkT8EkkhdgSkouUMmxuARTLGmJGJd0Am5SOmCMecXlNZhQwajEspqCbu3c4IML9dDpPsQT1L6G_d5GfbRD3xoYQ2dhaOCq9V674AYHP23Mar_6qE2fYOuhCV2nd9aj1teDsTXUsd_H9pxfLjbLuYGuNfYWXDW6S_buZ8_Ax_PT--oVbd5e1qvFBpmCsR5RUlImC8EwLQwxmsuG0kZLKUWVReLKFsLUXGx1abaWcFPXhbFU04YJzHUxA2TsNTGkFG2jjrF1Op4UwepMTo3kVCanzuSUzJmHMXMcts7Wf4lfVNlAR0PKJ7-zUR3CEH3-45_Wb86DesI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Immunomodulatory and therapeutic role of Cinnamomum verum extracts in collagen-induced arthritic BALB/c mice</title><source>Springer Nature</source><creator>Qadir, Mirza Muhammad Fahd ; Bhatti, Attya ; Ashraf, Muhammad Usman ; Sandhu, Mansur Abdullah ; Anjum, Sidrah ; John, Peter</creator><creatorcontrib>Qadir, Mirza Muhammad Fahd ; Bhatti, Attya ; Ashraf, Muhammad Usman ; Sandhu, Mansur Abdullah ; Anjum, Sidrah ; John, Peter</creatorcontrib><description><![CDATA[Ethnopharmacological relevance
Cinnamomum verum
(CV), also known as ‘Dalchini’, is the dry bark of the
Cinnamomum verum
(L.) plant, and has been used as a traditional Pakistani medicine to alleviate pain and inflammation in patients suffering from arthritic rheumatism. It contains alkaloids, triterpenes, Cinnamaldehyde and other volatile oils. The aim of the present study was to investigate the underlying biological effect of ethyl alcohol (EtOH) and methyl alcohol (MeOH) extracts from CV on collagen type-II induced arthritic (CIA) mice.
Materials and methods
Gas chromatography mass spectrophotometry was used to perform fingerprinting identification of the EtOH and MeOH extracts. CIA mice model was established by subdermal injections of type-II bovine collagen (CII) on the 1st, 8th and 14th day of the experiment. Ethyl alcohol extract and methyl alcohol extract (1 mg/KgBW, 2 mg/KgBW and 4 mg/KgBW), was orally administered from the 15th day onwards for 2 weeks. Progression of oedema and joint inflammation was measured in the paws using a digital Vernier calliper every 3 days from day 1 till the end of the experiment. The oxidative scavenging ability of cinnamaldehyde was evaluated using a DPPH assay. Similarly, the nitrogen free radical (NOS) production of isolated lymphocytes was evaluated using Greiss’s method. The spleen index was calculated and knee joint changes were observed by histopathological sectioning. Western blot analysis was performed on peripheral blood derived serum for CII, CAPN1, TNFα and NFATc3.
Results
Extracts were shown to be enriched in trans-cinnamaldehyde and its analogues. Extracts showed good ameliorative effects (
p
< 0.05) after day 2 of treatment. A greater therapeutic role was observed for the 4 mg/kgBW dosage of the methanolic extract (
p
< 0.01). Swelling in the spleen was greatly reduced along with the generation of free radicals by lymphocytes, post treatment. There was also an inhibitory role by the extracts on NFATc3 (p < 0.05), TNF-Alpha (p < 0.05), CAII (p < 0.05) and mCalpain (p < 0.05) all proteins involved in RA.
Conclusion
In this study, it has been demonstrated that administration of CV has a therapeutic potential on CIA. The data suggest that CV could have a potential role in the treatment of RA patients.]]></description><identifier>ISSN: 0925-4692</identifier><identifier>EISSN: 1568-5608</identifier><identifier>DOI: 10.1007/s10787-017-0349-9</identifier><identifier>PMID: 28429097</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Allergology ; Biomedical and Life Sciences ; Biomedicine ; Dermatology ; Gastroenterology ; Immunology ; Original Article ; Pharmacology/Toxicology ; Rheumatology</subject><ispartof>Inflammopharmacology, 2018-02, Vol.26 (1), p.157-170</ispartof><rights>Springer International Publishing 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-215249384023c1ca69f22fa99987999907e38cd68ba5cbe16cdd3ce2a2f4806a3</citedby><cites>FETCH-LOGICAL-c344t-215249384023c1ca69f22fa99987999907e38cd68ba5cbe16cdd3ce2a2f4806a3</cites><orcidid>0000-0002-6764-6151</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28429097$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qadir, Mirza Muhammad Fahd</creatorcontrib><creatorcontrib>Bhatti, Attya</creatorcontrib><creatorcontrib>Ashraf, Muhammad Usman</creatorcontrib><creatorcontrib>Sandhu, Mansur Abdullah</creatorcontrib><creatorcontrib>Anjum, Sidrah</creatorcontrib><creatorcontrib>John, Peter</creatorcontrib><title>Immunomodulatory and therapeutic role of Cinnamomum verum extracts in collagen-induced arthritic BALB/c mice</title><title>Inflammopharmacology</title><addtitle>Inflammopharmacol</addtitle><addtitle>Inflammopharmacology</addtitle><description><![CDATA[Ethnopharmacological relevance
Cinnamomum verum
(CV), also known as ‘Dalchini’, is the dry bark of the
Cinnamomum verum
(L.) plant, and has been used as a traditional Pakistani medicine to alleviate pain and inflammation in patients suffering from arthritic rheumatism. It contains alkaloids, triterpenes, Cinnamaldehyde and other volatile oils. The aim of the present study was to investigate the underlying biological effect of ethyl alcohol (EtOH) and methyl alcohol (MeOH) extracts from CV on collagen type-II induced arthritic (CIA) mice.
Materials and methods
Gas chromatography mass spectrophotometry was used to perform fingerprinting identification of the EtOH and MeOH extracts. CIA mice model was established by subdermal injections of type-II bovine collagen (CII) on the 1st, 8th and 14th day of the experiment. Ethyl alcohol extract and methyl alcohol extract (1 mg/KgBW, 2 mg/KgBW and 4 mg/KgBW), was orally administered from the 15th day onwards for 2 weeks. Progression of oedema and joint inflammation was measured in the paws using a digital Vernier calliper every 3 days from day 1 till the end of the experiment. The oxidative scavenging ability of cinnamaldehyde was evaluated using a DPPH assay. Similarly, the nitrogen free radical (NOS) production of isolated lymphocytes was evaluated using Greiss’s method. The spleen index was calculated and knee joint changes were observed by histopathological sectioning. Western blot analysis was performed on peripheral blood derived serum for CII, CAPN1, TNFα and NFATc3.
Results
Extracts were shown to be enriched in trans-cinnamaldehyde and its analogues. Extracts showed good ameliorative effects (
p
< 0.05) after day 2 of treatment. A greater therapeutic role was observed for the 4 mg/kgBW dosage of the methanolic extract (
p
< 0.01). Swelling in the spleen was greatly reduced along with the generation of free radicals by lymphocytes, post treatment. There was also an inhibitory role by the extracts on NFATc3 (p < 0.05), TNF-Alpha (p < 0.05), CAII (p < 0.05) and mCalpain (p < 0.05) all proteins involved in RA.
Conclusion
In this study, it has been demonstrated that administration of CV has a therapeutic potential on CIA. The data suggest that CV could have a potential role in the treatment of RA patients.]]></description><subject>Allergology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Dermatology</subject><subject>Gastroenterology</subject><subject>Immunology</subject><subject>Original Article</subject><subject>Pharmacology/Toxicology</subject><subject>Rheumatology</subject><issn>0925-4692</issn><issn>1568-5608</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kM1OAyEUhYnR2Fp9ADeGF8ACwzCwbBt_mjRxo2tCGaadZoAGZox9e2lGXbq45y7uOSe5HwD3BD8SjKt5IrgSFcIkT8EkkhdgSkouUMmxuARTLGmJGJd0Am5SOmCMecXlNZhQwajEspqCbu3c4IML9dDpPsQT1L6G_d5GfbRD3xoYQ2dhaOCq9V674AYHP23Mar_6qE2fYOuhCV2nd9aj1teDsTXUsd_H9pxfLjbLuYGuNfYWXDW6S_buZ8_Ax_PT--oVbd5e1qvFBpmCsR5RUlImC8EwLQwxmsuG0kZLKUWVReLKFsLUXGx1abaWcFPXhbFU04YJzHUxA2TsNTGkFG2jjrF1Op4UwepMTo3kVCanzuSUzJmHMXMcts7Wf4lfVNlAR0PKJ7-zUR3CEH3-45_Wb86DesI</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>Qadir, Mirza Muhammad Fahd</creator><creator>Bhatti, Attya</creator><creator>Ashraf, Muhammad Usman</creator><creator>Sandhu, Mansur Abdullah</creator><creator>Anjum, Sidrah</creator><creator>John, Peter</creator><general>Springer International Publishing</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-6764-6151</orcidid></search><sort><creationdate>20180201</creationdate><title>Immunomodulatory and therapeutic role of Cinnamomum verum extracts in collagen-induced arthritic BALB/c mice</title><author>Qadir, Mirza Muhammad Fahd ; Bhatti, Attya ; Ashraf, Muhammad Usman ; Sandhu, Mansur Abdullah ; Anjum, Sidrah ; John, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-215249384023c1ca69f22fa99987999907e38cd68ba5cbe16cdd3ce2a2f4806a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Allergology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Dermatology</topic><topic>Gastroenterology</topic><topic>Immunology</topic><topic>Original Article</topic><topic>Pharmacology/Toxicology</topic><topic>Rheumatology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qadir, Mirza Muhammad Fahd</creatorcontrib><creatorcontrib>Bhatti, Attya</creatorcontrib><creatorcontrib>Ashraf, Muhammad Usman</creatorcontrib><creatorcontrib>Sandhu, Mansur Abdullah</creatorcontrib><creatorcontrib>Anjum, Sidrah</creatorcontrib><creatorcontrib>John, Peter</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Inflammopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qadir, Mirza Muhammad Fahd</au><au>Bhatti, Attya</au><au>Ashraf, Muhammad Usman</au><au>Sandhu, Mansur Abdullah</au><au>Anjum, Sidrah</au><au>John, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunomodulatory and therapeutic role of Cinnamomum verum extracts in collagen-induced arthritic BALB/c mice</atitle><jtitle>Inflammopharmacology</jtitle><stitle>Inflammopharmacol</stitle><addtitle>Inflammopharmacology</addtitle><date>2018-02-01</date><risdate>2018</risdate><volume>26</volume><issue>1</issue><spage>157</spage><epage>170</epage><pages>157-170</pages><issn>0925-4692</issn><eissn>1568-5608</eissn><abstract><![CDATA[Ethnopharmacological relevance
Cinnamomum verum
(CV), also known as ‘Dalchini’, is the dry bark of the
Cinnamomum verum
(L.) plant, and has been used as a traditional Pakistani medicine to alleviate pain and inflammation in patients suffering from arthritic rheumatism. It contains alkaloids, triterpenes, Cinnamaldehyde and other volatile oils. The aim of the present study was to investigate the underlying biological effect of ethyl alcohol (EtOH) and methyl alcohol (MeOH) extracts from CV on collagen type-II induced arthritic (CIA) mice.
Materials and methods
Gas chromatography mass spectrophotometry was used to perform fingerprinting identification of the EtOH and MeOH extracts. CIA mice model was established by subdermal injections of type-II bovine collagen (CII) on the 1st, 8th and 14th day of the experiment. Ethyl alcohol extract and methyl alcohol extract (1 mg/KgBW, 2 mg/KgBW and 4 mg/KgBW), was orally administered from the 15th day onwards for 2 weeks. Progression of oedema and joint inflammation was measured in the paws using a digital Vernier calliper every 3 days from day 1 till the end of the experiment. The oxidative scavenging ability of cinnamaldehyde was evaluated using a DPPH assay. Similarly, the nitrogen free radical (NOS) production of isolated lymphocytes was evaluated using Greiss’s method. The spleen index was calculated and knee joint changes were observed by histopathological sectioning. Western blot analysis was performed on peripheral blood derived serum for CII, CAPN1, TNFα and NFATc3.
Results
Extracts were shown to be enriched in trans-cinnamaldehyde and its analogues. Extracts showed good ameliorative effects (
p
< 0.05) after day 2 of treatment. A greater therapeutic role was observed for the 4 mg/kgBW dosage of the methanolic extract (
p
< 0.01). Swelling in the spleen was greatly reduced along with the generation of free radicals by lymphocytes, post treatment. There was also an inhibitory role by the extracts on NFATc3 (p < 0.05), TNF-Alpha (p < 0.05), CAII (p < 0.05) and mCalpain (p < 0.05) all proteins involved in RA.
Conclusion
In this study, it has been demonstrated that administration of CV has a therapeutic potential on CIA. The data suggest that CV could have a potential role in the treatment of RA patients.]]></abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>28429097</pmid><doi>10.1007/s10787-017-0349-9</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-6764-6151</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0925-4692 |
| ispartof | Inflammopharmacology, 2018-02, Vol.26 (1), p.157-170 |
| issn | 0925-4692 1568-5608 |
| language | eng |
| recordid | cdi_crossref_primary_10_1007_s10787_017_0349_9 |
| source | Springer Nature |
| subjects | Allergology Biomedical and Life Sciences Biomedicine Dermatology Gastroenterology Immunology Original Article Pharmacology/Toxicology Rheumatology |
| title | Immunomodulatory and therapeutic role of Cinnamomum verum extracts in collagen-induced arthritic BALB/c mice |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T01%3A59%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immunomodulatory%20and%20therapeutic%20role%20of%20Cinnamomum%20verum%20extracts%20in%20collagen-induced%20arthritic%20BALB/c%20mice&rft.jtitle=Inflammopharmacology&rft.au=Qadir,%20Mirza%20Muhammad%20Fahd&rft.date=2018-02-01&rft.volume=26&rft.issue=1&rft.spage=157&rft.epage=170&rft.pages=157-170&rft.issn=0925-4692&rft.eissn=1568-5608&rft_id=info:doi/10.1007/s10787-017-0349-9&rft_dat=%3Cpubmed_cross%3E28429097%3C/pubmed_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c344t-215249384023c1ca69f22fa99987999907e38cd68ba5cbe16cdd3ce2a2f4806a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/28429097&rfr_iscdi=true |