Appraisal of disease-modifying potential of amlodipine as an anti-arthritic agent: new indication for an old drug

Amlodipine, a second-generation calcium channel blocker, exhibits documented anti-inflammatory potential. Thereby, present investigation was accomplished with an aim to explore anti-arthritic potential of amlodipine, giving a second chance to an existing drug. For validation of anti-arthritic potent...

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Bibliographic Details
Published in:Inflammopharmacology 2020-08, Vol.28 (4), p.1121-1136
Main Authors: Qasim, Sumera, Alamgeer, Kalsoom, Saima, Shahzad, Muhammad, Irfan, Hafiz Muhammad, Zafar, Muhammad Shoaib, Bukhari, Ishfaq Ali, Vohra, Fahim, Afzal, Sibtain
Format: Article
Language:English
Subjects:
Albumins - metabolism
Allergology
Amlodipine - pharmacology
Animals
Antirheumatic Agents - pharmacology
Arthritis, Experimental - diagnostic imaging
Arthritis, Experimental - drug therapy
Arthritis, Experimental - genetics
Arthritis, Experimental - metabolism
Biomedical and Life Sciences
Biomedicine
Cytokines - biosynthesis
Cytokines - genetics
Dermatology
Drug Repositioning
Erythrocytes - drug effects
Female
Gastroenterology
Gene Expression - drug effects
Humans
Immunology
Male
Original Article
Pharmacology/Toxicology
Rats
Rheumatology
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Summary:Amlodipine, a second-generation calcium channel blocker, exhibits documented anti-inflammatory potential. Thereby, present investigation was accomplished with an aim to explore anti-arthritic potential of amlodipine, giving a second chance to an existing drug. For validation of anti-arthritic potential of amlodipine, some in vitro models comprised of bovine serum albumin- and egg albumin-induced protein denaturation along with membrane stabilization of red blood cell was being conducted. In vivo models comprised of formaldehyde-provoked acute arthritis and CFA-instigated chronic arthritic. Paw edema, arthritic index, body weight alterations, biochemical and hematological parameters, and ankle joint histological and radiographic investigations were appraised. Moreover, RT-PCR was conducted to evaluate the levels of several inflammatory markers. Molecular docking was being conducted targeting TNF-α, IL-1β and IL-6 to establish the correlation between experimental and theoretical results. Amlodipine provides significant protection against denaturation being provoked by heating egg albumin and BSA along with stabilizing membrane of red blood cell, thereby proving in vitro anti-arthritic effect. A significant ( p  
ISSN:0925-4692
1568-5608
DOI:10.1007/s10787-020-00692-9