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Distribution of fluorescence decay times for 1-anilinonaphthalene-8-sulfonate in human oxyhemoglobin A1b solution
We have studied complex formation between molecules of the fluorescent probe 1-anilinonaphthalene-8-sulfonate (1,8-ANS) and the major form (A 1 ) and a minor form (A b ) of hemoglobin. The contribution of the longlived component f 3 to the kinetic curves for fluorescence decay in HbA 1b solutions is...
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| Published in: | Journal of applied spectroscopy 2012-07, Vol.79 (3), p.437-445 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| container_end_page | 445 |
| container_issue | 3 |
| container_start_page | 437 |
| container_title | Journal of applied spectroscopy |
| container_volume | 79 |
| creator | Drazdou, A. S. Sobchuk, A. N. Syakhovich, V. E. Bokut, O. S. Kvasyuk, E. I. Bushuk, B. A. Bokut, S. B. |
| description | We have studied complex formation between molecules of the fluorescent probe 1-anilinonaphthalene-8-sulfonate (1,8-ANS) and the major form (A
1
) and a minor form (A
b
) of hemoglobin. The contribution of the longlived component f
3
to the kinetic curves for fluorescence decay in HbA
1b
solutions is 0.021–0.036, which indicates a dramatic decrease (compared with HbA
1
) in the accessibility of the central cavity of HbA
1b
for binding 1,8-ANS. Disappearance of the long-lived component f
3
in the fluorescence decay kinetics of 1,8-ANS in HbA
1b
solutions in the presence of inositol hexaphosphate (IHP) suggests that the regulatory region of HbA1b is completely inaccessible for interaction both with the negatively charged molecules of the probe and with natural regulators of the transport function for this form of the heme protein. |
| doi_str_mv | 10.1007/s10812-012-9620-4 |
| format | article |
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1
) and a minor form (A
b
) of hemoglobin. The contribution of the longlived component f
3
to the kinetic curves for fluorescence decay in HbA
1b
solutions is 0.021–0.036, which indicates a dramatic decrease (compared with HbA
1
) in the accessibility of the central cavity of HbA
1b
for binding 1,8-ANS. Disappearance of the long-lived component f
3
in the fluorescence decay kinetics of 1,8-ANS in HbA
1b
solutions in the presence of inositol hexaphosphate (IHP) suggests that the regulatory region of HbA1b is completely inaccessible for interaction both with the negatively charged molecules of the probe and with natural regulators of the transport function for this form of the heme protein.</description><identifier>ISSN: 0021-9037</identifier><identifier>EISSN: 1573-8647</identifier><identifier>DOI: 10.1007/s10812-012-9620-4</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Analytical Chemistry ; Atomic/Molecular Structure and Spectra ; Physics ; Physics and Astronomy</subject><ispartof>Journal of applied spectroscopy, 2012-07, Vol.79 (3), p.437-445</ispartof><rights>Springer Science+Business Media, Inc. 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1554-ad2ba60feb010937793dd26258cc1becefbbd7c0c1a2caf21406e6f4f9ca32833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><creatorcontrib>Drazdou, A. S.</creatorcontrib><creatorcontrib>Sobchuk, A. N.</creatorcontrib><creatorcontrib>Syakhovich, V. E.</creatorcontrib><creatorcontrib>Bokut, O. S.</creatorcontrib><creatorcontrib>Kvasyuk, E. I.</creatorcontrib><creatorcontrib>Bushuk, B. A.</creatorcontrib><creatorcontrib>Bokut, S. B.</creatorcontrib><title>Distribution of fluorescence decay times for 1-anilinonaphthalene-8-sulfonate in human oxyhemoglobin A1b solution</title><title>Journal of applied spectroscopy</title><addtitle>J Appl Spectrosc</addtitle><description>We have studied complex formation between molecules of the fluorescent probe 1-anilinonaphthalene-8-sulfonate (1,8-ANS) and the major form (A
1
) and a minor form (A
b
) of hemoglobin. The contribution of the longlived component f
3
to the kinetic curves for fluorescence decay in HbA
1b
solutions is 0.021–0.036, which indicates a dramatic decrease (compared with HbA
1
) in the accessibility of the central cavity of HbA
1b
for binding 1,8-ANS. Disappearance of the long-lived component f
3
in the fluorescence decay kinetics of 1,8-ANS in HbA
1b
solutions in the presence of inositol hexaphosphate (IHP) suggests that the regulatory region of HbA1b is completely inaccessible for interaction both with the negatively charged molecules of the probe and with natural regulators of the transport function for this form of the heme protein.</description><subject>Analytical Chemistry</subject><subject>Atomic/Molecular Structure and Spectra</subject><subject>Physics</subject><subject>Physics and Astronomy</subject><issn>0021-9037</issn><issn>1573-8647</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp9kM1KQzEQhYMoWKsP4C4vEJ3k_i9L_YWCG12HJHfSm3Kb1OResG9vtK5dDAOHOWcOHyG3HO44QHOfOLRcMMjT1QJYeUYWvGoK1tZlc04WAIKzDormklyltAOArhWwIJ8PLk3R6XlywdNgqR3nEDEZ9AZpj0Yd6eT2mKgNkXKmvBudD14dhmlQI3pkLUvzaLM0IXWeDvNe5aSv44D7sB2DztqKa5rC-PvkmlxYNSa8-dtL8vH0-L5-YZu359f1asMMr6qSqV5oVYNFDRy6omm6ou9FLarWGK7RoNW6bwwYroRRVvASaqxtaTujCtEWxZLwU66JIaWIVh6i26t4lBzkDzN5YiYzM_nDTJbZI06elG_9FqPchTn6XPMf0zf9EXIL</recordid><startdate>20120701</startdate><enddate>20120701</enddate><creator>Drazdou, A. S.</creator><creator>Sobchuk, A. N.</creator><creator>Syakhovich, V. E.</creator><creator>Bokut, O. S.</creator><creator>Kvasyuk, E. I.</creator><creator>Bushuk, B. A.</creator><creator>Bokut, S. B.</creator><general>Springer US</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20120701</creationdate><title>Distribution of fluorescence decay times for 1-anilinonaphthalene-8-sulfonate in human oxyhemoglobin A1b solution</title><author>Drazdou, A. S. ; Sobchuk, A. N. ; Syakhovich, V. E. ; Bokut, O. S. ; Kvasyuk, E. I. ; Bushuk, B. A. ; Bokut, S. B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1554-ad2ba60feb010937793dd26258cc1becefbbd7c0c1a2caf21406e6f4f9ca32833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analytical Chemistry</topic><topic>Atomic/Molecular Structure and Spectra</topic><topic>Physics</topic><topic>Physics and Astronomy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Drazdou, A. S.</creatorcontrib><creatorcontrib>Sobchuk, A. N.</creatorcontrib><creatorcontrib>Syakhovich, V. E.</creatorcontrib><creatorcontrib>Bokut, O. S.</creatorcontrib><creatorcontrib>Kvasyuk, E. I.</creatorcontrib><creatorcontrib>Bushuk, B. A.</creatorcontrib><creatorcontrib>Bokut, S. B.</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of applied spectroscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Drazdou, A. S.</au><au>Sobchuk, A. N.</au><au>Syakhovich, V. E.</au><au>Bokut, O. S.</au><au>Kvasyuk, E. I.</au><au>Bushuk, B. A.</au><au>Bokut, S. B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of fluorescence decay times for 1-anilinonaphthalene-8-sulfonate in human oxyhemoglobin A1b solution</atitle><jtitle>Journal of applied spectroscopy</jtitle><stitle>J Appl Spectrosc</stitle><date>2012-07-01</date><risdate>2012</risdate><volume>79</volume><issue>3</issue><spage>437</spage><epage>445</epage><pages>437-445</pages><issn>0021-9037</issn><eissn>1573-8647</eissn><abstract>We have studied complex formation between molecules of the fluorescent probe 1-anilinonaphthalene-8-sulfonate (1,8-ANS) and the major form (A
1
) and a minor form (A
b
) of hemoglobin. The contribution of the longlived component f
3
to the kinetic curves for fluorescence decay in HbA
1b
solutions is 0.021–0.036, which indicates a dramatic decrease (compared with HbA
1
) in the accessibility of the central cavity of HbA
1b
for binding 1,8-ANS. Disappearance of the long-lived component f
3
in the fluorescence decay kinetics of 1,8-ANS in HbA
1b
solutions in the presence of inositol hexaphosphate (IHP) suggests that the regulatory region of HbA1b is completely inaccessible for interaction both with the negatively charged molecules of the probe and with natural regulators of the transport function for this form of the heme protein.</abstract><cop>Boston</cop><pub>Springer US</pub><doi>10.1007/s10812-012-9620-4</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of applied spectroscopy, 2012-07, Vol.79 (3), p.437-445 |
| issn | 0021-9037 1573-8647 |
| language | eng |
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| source | Springer Nature |
| subjects | Analytical Chemistry Atomic/Molecular Structure and Spectra Physics Physics and Astronomy |
| title | Distribution of fluorescence decay times for 1-anilinonaphthalene-8-sulfonate in human oxyhemoglobin A1b solution |
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