A Highly Sensitive Fluorescent Probe for HClO and Its Application in Live Cell Imaging

A new rhodamine-based probe 1 was designed and synthesized as a new fluorescent molecular probe for HClO in PBS buffer at physiological condition. The free probe 1 almost nonfluorescence, however, a drastic enhancement of fluorescence intensity was observed in the presence of HClO. The new probe 1 e...

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Bibliographic Details
Published in:Journal of fluorescence 2012-09, Vol.22 (5), p.1201-1207
Main Authors: Zuo, Qing-Ping, Li, Zuo-Jun, Hu, Yong-Hua, Li, Bin, Huang, Li-Hua, Wang, Chun-Jiang, Liu, Shi-Kun, Liao, Hai-Qiang
Format: Article
Language:English
Subjects:
Absorption
Analytical Chemistry
Animals
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biomedicine
Biophysics
Biotechnology
Buffers
Cell Line
Cell Membrane Permeability
Cell Survival
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Fluorescent Dyes - metabolism
Hydrogen-Ion Concentration
Hypochlorous Acid - metabolism
Mice
Molecular Imaging - methods
Rhodamines - chemical synthesis
Rhodamines - chemistry
Rhodamines - metabolism
Short Communication
Spectrometry, Fluorescence
Time Factors
Water - chemistry
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Summary:A new rhodamine-based probe 1 was designed and synthesized as a new fluorescent molecular probe for HClO in PBS buffer at physiological condition. The free probe 1 almost nonfluorescence, however, a drastic enhancement of fluorescence intensity was observed in the presence of HClO. The new probe 1 exhibits good sensitivity and selectivity for HClO over other reactive oxygen and/or nitrogen species in PBS buffer, and the probe was successfully applied to image endogeneous HClO in the living cells.
ISSN:1053-0509
1573-4994
DOI:10.1007/s10895-012-1067-8