Studies on 99mTc-labeling of the modified fragment of human alfa-fetoprotein (P149-QY)

The present study shows the development of 99m Tc-labeling of the P149-QY peptide, a modified fragment of human alpha-fetoprotein (hAFP), with the intention of potential application for diagnosis of AFP positive tumors. An efficient preparation of 99m Tc-labeled P149-QY using HYNIC was developed. It...

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Bibliographic Details
Published in:Journal of radioanalytical and nuclear chemistry 2008, Vol.275 (1), p.101-107
Main Authors: Maurin, M., Garnuszek, P., Karczmarczyk, U., Mirowski, M.
Format: Article
Language:English
Subjects:
Chemistry
Chemistry and Materials Science
Diagnostic Radiology
Hadrons
Heavy Ions
Inorganic Chemistry
Nuclear Chemistry
Nuclear Physics
Physical Chemistry
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Summary:The present study shows the development of 99m Tc-labeling of the P149-QY peptide, a modified fragment of human alpha-fetoprotein (hAFP), with the intention of potential application for diagnosis of AFP positive tumors. An efficient preparation of 99m Tc-labeled P149-QY using HYNIC was developed. It has been shown that the free sulfhydryl groups in P149-QY can be protected with N-ethylmaleimide, preventing the radiolabeled peptide from loss of its biological activity. The biodistribution studies revealed favorable pharmacokinetics and a better specificity to the mammary adenocarcinoma tumor for the SH-protected radiolabeled P149-QY peptide in comparison to the radioiodinated and to the directly 99m Tc-labeled peptide.
ISSN:0236-5731
1588-2780
DOI:10.1007/s10967-007-6980-y