Characterization of the composition and antioxidant activity of plant extracts by HPLC with UV and amperometric detection

High-performance liquid chromatography with UV and amperometric detection has been used to analyze qualitatively and quantitatively phenolic extracts of several plants used in the production of drugs, food, and cosmetics. Phenolic compounds in aqueous alcohol, alcohol, and propyleneglycol (PPG) extr...

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Bibliographic Details
Published in:Pharmaceutical chemistry journal 2008-09, Vol.42 (9), p.539-544
Main Authors: Semenistaya, E. N, Larionov, O. G
Format: Article
Language:English
Subjects:
Medicinal Plants
Medicine
Organic Chemistry
Pharmacology/Toxicology
Pharmacy
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Summary:High-performance liquid chromatography with UV and amperometric detection has been used to analyze qualitatively and quantitatively phenolic extracts of several plants used in the production of drugs, food, and cosmetics. Phenolic compounds in aqueous alcohol, alcohol, and propyleneglycol (PPG) extracts were identified as groups using a previously constructed database of the spectra and retention times of 74 phenolic compounds. The antioxidant activity (AA) of the extracts was measured as the sum of peaks on the chromatograms after the amperometric detector. The AA of the alcohol extracts correlated with the flavonoid content. Extracts obtained using supercritical PPG had the maximum AA.
ISSN:0091-150X
1573-9031
DOI:10.1007/s11094-009-0167-5