Ascorbate plays a key role in alleviating low temperature-induced oxidative stress in Arabidopsis

• Published in: Photosynthetica 2012-12, Vol.50 (4), p.602-612
• Main Authors: Wang, L. Y, Zhang, Q. Y, Wang, F, Meng, X, Meng, Q. W
• Format: Article
• Language: English
• Subjects: antioxidant activity; antioxidants; Arabidopsis thaliana; ascorbate peroxidase; Biomedical and Life Sciences; cells; chlorophyll; glutathione; hydrogen peroxide; Life Sciences; mutants; oxidative stress; photosystem II; Plant Physiology; temperature; thiobarbituric acid-reactive substances
• ISSN: 0300-3604; 1573-9058
• DOI: 10.1007/s11099-012-0072-0

Low temperature has a negative impact on plant cells and results in the generation of reactive oxygen species (ROS). In order to study the role of ascorbate under chilling stress, the response of an ascorbate-deficient Arabidopsis thaliana mutant vtc2-1 to low temperature (2°C) was investigated. After chilling stress, vtc2-1 mutants exhibited oxidative damage. An increase in the H₂O₂ generation and the production of thiobarbituric acid reactive substances (TBARS), and a decrease in chlorophyll content, the maximal photochemical efficiency of PSII (Fᵥ/Fₘ) and oxidizable P₇₀₀ were also noted. The ratio of ascorbate/dehydroascorbate and reduced glutathione/oxidzed glutathione in the vtc2-1 mutants were reduced, compared with the wild type (WT) plants. The activities of antioxidant enzymes, such as catalase (CAT) and ascorbate peroxidase (APX), and soluble antioxidants were lower in the vtc2-1 mutants than those in WT plants. These results suggested that the ascorbate-deficient mutant vtc2-1 was more sensitive to chilling treatment than WT plants. The low temperature-induced oxidative stress was the major cause of the decrease of PSII and PSI function in the vtc2-1 mutants. Ascorbate plays a critical role of defense without which the rest of the ROS defense network is unable to react effectively.