Wounding and medium formulation affect de novo shoot organogenic responses in hypocotyl-derived explants of annatto (Bixa orellana L.)

• Published in: In vitro cellular & developmental biology. Plant 2019-06, Vol.55 (3), p.277-289
• Main Authors: Faria, Daniele Vidal, de Freitas Correia, Ludmila Nayara, de Matos, Elyabe Monteiro, de Souza, Maria Victoria Costa, Batista, Diego Silva, Costa, Marcio Gilberto Cardoso, de Paiva Neto, Vespasiano Borges, Xavier, Aloisio, Rogalski, Marcelo, Otoni, Wagner Campos
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Cell Biology; Developmental Biology; Life Sciences; Micropropagation; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant Sciences
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/s11627-019-09978-2

Bixa orellana L. (annatto) is the unique exploited source for the production of bixin and norbixin natural dyes, which are widely used in the food, pharmaceutical, cosmetic, and textile industries. In tissue culture, adequate culture conditions are essential for the development of the plant. Among them, the saline composition of the culture medium and the type of explants are limiting factors. In this study, the effect of MS (Murashige and Skoog), Correia and colleagues (JADS) medium, Woody Plant Medium (WPM), and Driver Kuniyuki Walnut (DKW) culture media and wounding of hypocotyl explants on de novo shoot organogenesis (DNSO) of annatto were evaluated. It was demonstrated that JADS medium improved the nutritional status of shoots, which promoted the best development and quality of the plants generated after acclimatization, when compared to MS medium. The use of JADS medium for annatto DNSO during 45 d of culture was sufficient to regenerate and stimulate the growth of shoots, which had adequate elongation and rhizogenic responses. In addition, enlargement of the wound surface, using longitudinal sections of the hypocotyl explant cut surface down on JADS medium, significantly improved the frequency and number of newly formed shoots. This protocol enabled production of an average of 17 30-mm-long shoots per explant after 90 d of culture. These results will be useful for future studies related to annatto biotechnology.