Qianliening capsule (前列宁胶囊) inhibits human prostate cell growth via induction of mitochondrion-dependent cell apoptosis

Objective To investigate the molecular mechanisms by which Qianliening Capsule (前列宁胶囊, QC) treats benign prostatic hyperplasia (BPH). Methods Human prostate stromal cell line WPMY-1 was treated with 0, 1, 3 and 5 mg/mL of QC for 24, 48 and 72 h, respectively, in the presence of 10 ng/mL basic fibrob...

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Bibliographic Details
Published in:Chinese journal of integrative medicine 2012-11, Vol.18 (11), p.824-830
Main Authors: Hong, Zhen-feng, Lin, Jiu-mao, Zhong, Xiao-yong, Li, Ying, Zhou, Jian-heng, Xu, Wei, Peng, Jun
Format: Article
Language:English
Subjects:
Antineoplastic Agents, Phytogenic - administration & dosage
Antineoplastic Agents, Phytogenic - pharmacology
Apoptosis - drug effects
Capsules
Cell Proliferation - drug effects
Cell Survival - drug effects
Cells, Cultured
Down-Regulation - drug effects
Drug Evaluation, Preclinical
Drugs, Chinese Herbal - administration & dosage
Drugs, Chinese Herbal - pharmacology
Humans
Male
Medicine
Medicine & Public Health
Membrane Potential, Mitochondrial - drug effects
Mitochondria - drug effects
Mitochondria - physiology
Original Article
Prostate - cytology
Prostate - drug effects
Prostate - physiology
Stromal Cells - drug effects
Stromal Cells - physiology
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Summary:Objective To investigate the molecular mechanisms by which Qianliening Capsule (前列宁胶囊, QC) treats benign prostatic hyperplasia (BPH). Methods Human prostate stromal cell line WPMY-1 was treated with 0, 1, 3 and 5 mg/mL of QC for 24, 48 and 72 h, respectively, in the presence of 10 ng/mL basic fibroblast growth factor (bFGF). The viability of WPMY-1 cells was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell morphology was observed by phase-contrast microscopy. 4′,6-diamidino-2-phenylindole (DAPI) staining and fluorescence activated cell sorting (FACS) analysis with Annexin-V/propidium iodide (PI) staining were performed to determine cell apoptosis. The loss of mitochondrial membrane potential was examined by FACS analysis with 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolyl-carbocyarine iodide (JC-1) staining. Activation of caspase-3 and -9 was evaluated by colorimetric assay. The mRNA and protein expression levels of Bcl-2 and Bax were measured by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting, respectively. Results Upon bFGF stimulation, the viability of WPMY-1 cells was increased to 122%–118% compared with the control cells ( P
ISSN:1672-0415
1993-0402
DOI:10.1007/s11655-012-1264-y