Effects of Herbal Compound 861 on Collagen Synthesis and Degradation in Rat Mesangial Cells Exposed to High Glucose

• Published in: Chinese journal of integrative medicine 2014-03, Vol.20 (3), p.209-215
• Main Authors: Ma, Qing, Zhang, Li, Yao, Lan, Chen, Hai-ping, Wang, Bao-en
• Format: Article
• Language: English
• Subjects: Animals; Cell Proliferation - drug effects; Cells, Cultured; Collagen Type IV - biosynthesis; Collagen Type IV - secretion; Drugs, Chinese Herbal - pharmacology; Fibrosis; Glucose - toxicity; Hepatocyte Growth Factor - secretion; Matrix Metalloproteinase 9 - metabolism; Medicine; Medicine & Public Health; Mesangial Cells - cytology; Mesangial Cells - drug effects; Mesangial Cells - enzymology; Mesangial Cells - metabolism; Original Article; Polymerase Chain Reaction; Proteolysis - drug effects; Rats; RNA, Messenger - genetics; RNA, Messenger - metabolism; TIMP-1; Tissue Inhibitor of Metalloproteinase-1 - metabolism; Transforming Growth Factor beta1 - secretion; 中药复方; 合成化合物; 大鼠; 肾小球系膜细胞; 胶原合成; 胶原降解; 高糖
• ISSN: 1672-0415; 1993-0402
• DOI: 10.1007/s11655-014-1741-6

Objective： To investigate the effects of Herbal Compound 861 （Cpd 861） on collagen synthesis and degradation in rat mesangial cells exposed to high glucose. Methods： The third to fifth passage of rat mesangial cells were exposed to high glucose and Cpd 861 at a concentration of 0.25-4.00 g/L for 24, 48 and 72 h, respectively. Benazepril （107-10s mmol/L） was selected as positive control. The methyl thiazolyl tetrazolium colorimetric assay was used to evaluate the effect of Cpd 861 on cell proliferation. After incubation with Cpd 861 at a concentration of 2.00 g/L for 48 h, the protein secretions of collagen type IV, matrix metallopeptidase 9 （MMP-9）, tissue inhibitor of metalloproteinase-1 （TIMP-1）, transforming growth factor beta 1 （TGF-131）, and hepatocyte growth factor （HGF） were detected by enzyme-linked immunosorbent assay method. And rat mesangial cells were harvested to determine MMP-9, TIMP-1, TGF-131 and HGF mRNA expression by revers~transcription polymerase chain reaction. Results： Cpd 861 inhibited cell proliferation induced by high glucose in a dose- and time-dependent manner. Compared with high glucose, collagen type IV production was decreased significantly by Cpd 861 （P〈0.01）. Cpd 861 increased the protein secretions and mRNA expressions of MMP-9 and HGF, whereas the protein secretions and mRNA expressions of TIMP-1 and TGF-131 were reduced markedly （P〈0.05）. The ratio of MMP-9 to TIMP-1 was enhanced by Cpd 861 significantly. There was no significant difference in all above-mentioned effects between Cpd 861 （2.00 g/L） and benazepril （10-5 mmol/L）. Conclusion： The anti-glomerulosclerosis mechanisms of Cpd 861 were partly attributed to its effects of inhibiting mesangial cell proliferation, decreasing collagen synthesis and enhancing collagen degradation.