Development of two microsatellite multiplex PCR systems for high throughput genotyping in Populus euphratica

Eighteen microsatellite primer pairs previously developed at Oak Ridge National Laboratory for Populus tremuloides Michx. and Populus trichocarpa Tort. ＆ Gray were screened for amplification in Euphrates poplar, Populus euphratica Oliv. Thirteen loci were found to express polymorphisms ranging from...

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Bibliographic Details
Published in:Journal of forestry research 2009, Vol.20 (3), p.195-198
Main Authors: Eusemann, Pascal, Fehrenz, Steffen, Schnittler, Martin
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Forestry
Life Sciences
PCR技术
Research Paper
吞吐量
国家实验室
基因分型
微卫星引物
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Summary:Eighteen microsatellite primer pairs previously developed at Oak Ridge National Laboratory for Populus tremuloides Michx. and Populus trichocarpa Tort. ＆ Gray were screened for amplification in Euphrates poplar, Populus euphratica Oliv. Thirteen loci were found to express polymorphisms ranging from two to 17 alleles. The eight most variable loci were selected to set up and optimize two multiplex polymerase chain reaction （PCR） assays. Three populations containing altogether 436 trees were used to characterize the selected loci and ascertain their applicability for parentage analysis and genotyping studies. Through cross-checking of clonal identity against sex of the genotyped trees we estimated the maximum error rate for merging genotypes to be less than 0.045.
ISSN:1007-662X
1993-0607
DOI:10.1007/s11676-009-0038-7