High frequency in vitro propagation of Isodon wightii (Bentham) H. Hara

A protocol for in vitro propagation of Isodon wightii (Bentham) H. Hara from nodal segments was developed. Multiple shoots were successfully established on half strength MS medium supplemented with 4.4 μM BA. Enhancement of shoot multiplication and elongation was achieved on half strength MS medium...

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Bibliographic Details
Published in:Acta physiologiae plantarum 2010, Vol.32 (2), p.405-409
Main Authors: Thirugnanasampandan, R, Mahendran, G, Narmatha Bai, V
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Life Sciences
Plant Anatomy/Development
Plant Biochemistry
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Short Communication
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Summary:A protocol for in vitro propagation of Isodon wightii (Bentham) H. Hara from nodal segments was developed. Multiple shoots were successfully established on half strength MS medium supplemented with 4.4 μM BA. Enhancement of shoot multiplication and elongation was achieved on half strength MS medium supplemented with 4.4 μM BA and 1.4 μM GA₃. The regenerated shoots were rooted successfully on half strength MS medium supplemented with 4.9 μM IBA. Acclimatization of in vitro rooted shoots was successful. The in vitro regenerated plants grew well in the greenhouse without any phenotypic changes.
ISSN:0137-5881
1861-1664
DOI:10.1007/s11738-009-0401-5