Caffeic acid derivatives from a hairy root culture of Lactuca virosa

In a search for biologically active phenolics, a hydroalcoholic extract from the hairy roots of Lactuca virosa was fractionated by chromatographical methods. The procedure led to the isolation of a substantial amount of 3,5-dicaffeoylquinic acid (3,5-DCQA)—a potent free radical scavenger. An analyti...

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Bibliographic Details
Published in:Acta physiologiae plantarum 2012, Vol.34 (1), p.291-298
Main Authors: Stojakowska, Anna, Malarz, Janusz, Szewczyk, Agnieszka, Kisiel, Wanda
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
caffeic acid
caftaric acid
callus culture
chlorogenic acid
free radical scavengers
Lactuca virosa
Life Sciences
Original Paper
Plant Anatomy/Development
Plant Biochemistry
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
reversed-phase high performance liquid chromatography
roots
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Summary:In a search for biologically active phenolics, a hydroalcoholic extract from the hairy roots of Lactuca virosa was fractionated by chromatographical methods. The procedure led to the isolation of a substantial amount of 3,5-dicaffeoylquinic acid (3,5-DCQA)—a potent free radical scavenger. An analytical RP-HPLC separation of the hydroalcoholic extract from the hairy roots allowed identification of further hydroxycinnamates: caftaric acid (CTA), chlorogenic acid (5-CQA) and cichoric acid (DCTA), as well as small amounts of unbound phenolic acids. A time course of growth and caffeic acid derivatives accumulation in the hairy root culture was also investigated. The highest contents of the compounds in the examined roots were detected at the logarithmic phase of growth. The average content of 3,5-DCQA in the roots (ca. 2.5% DW) was at least one order of magnitude higher than that found in roots of Lactuca species and callus culture of L. virosa.
ISSN:0137-5881
1861-1664
DOI:10.1007/s11738-011-0827-4