Comparative GC–FID and GC–MS analysis of the mono and sesquiterpene secondary metabolites produced by the field grown and micropropagated plants of Artemisia amygdalina Decne

• Published in: Acta physiologiae plantarum 2012-05, Vol.34 (3), p.885-890
• Main Authors: Rather, Manzoor A., Ganai, Bashir A., Kamili, Azra N., Qayoom, Mubashir, Akbar, Seema, Masood, Akbar, Rasool, Rafia, Wani, Sajad H., Qurishi, Mushtaq A.
• Format: Article
• Language: English
• Subjects: Agriculture; Biomedical and Life Sciences; Life Sciences; Original Paper; Plant Anatomy/Development; Plant Biochemistry; Plant Genetics and Genomics; Plant Pathology; Plant Physiology
• ISSN: 0137-5881; 1861-1664
• DOI: 10.1007/s11738-011-0886-6

The essential oil obtained by hydrodistillation from the leaves of micropropagated plants of Artemisia amygdalina was analyzed by capillary GC–FID and GC–MS and compared with that obtained from the leaves of field growing parent plants. The oil yield from the micropropagated plants was lower (0.05% v/w) than the oil yield obtained from field-grown plants (0.2% v/w). The major constituents of the field-grown plants were p -cymene (21.0%), 1,8-cineole (24.9%), α-terpineol (5.9%), β-caryophyllene (4.7%), germacrene D (4.0%), while as the major constituents from the micropropagated plants were p -cymene (11.3%),1,8-cineole (10.2%), borneol (7.9%), α-longipinene (5.5%), α-copaene (5.5%) and β-caryophyllene (17%). The essential oil from field-grown plant was dominated by the presence of oxygenated monoterpenes (41.5%), monoterpene hydrocarbons (35.9%) and sesquiterpene hydrocarbons (16.3%) while as the essential oil of micropropagated plants was characterized by sesquiterpene hydrocarbons (40.0%), oxygenated monoterpenes (25.2%) and monoterpene hydrocarbons (21.6%).