Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level

Shoot cultures of rare Balkan hyperaccumulating species Alyssum markgrafii were subjected to high nickel concentrations of 1, 3, 5, and 8 mM. The effects of graded nickel concentrations on toxicity, pigments, and several components of plant antioxidative defense system were characterized. Toxic effe...

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Bibliographic Details
Published in:Acta physiologiae plantarum 2012-09, Vol.34 (5), p.1997-2006
Main Authors: Stanisavljević, Nemanja, Savić, Jelena, Jovanović, Živko, Miljuš-Djukić, Jovanka, Radović, Svetlana, Vinterhalter, Dragan, Vinterhalter, Branka
Format: Article
Language:English
Subjects:
Agriculture
Alyssum
antioxidants
Biomedical and Life Sciences
chlorophyll
enzyme activity
enzymes
hyperaccumulators
Life Sciences
lipid peroxidation
molecular weight
nickel
Original Paper
phenols
pigments
Plant Anatomy/Development
Plant Biochemistry
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
reactive oxygen species
shoots
sodium benzoate
toxicity
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Summary:Shoot cultures of rare Balkan hyperaccumulating species Alyssum markgrafii were subjected to high nickel concentrations of 1, 3, 5, and 8 mM. The effects of graded nickel concentrations on toxicity, pigments, and several components of plant antioxidative defense system were characterized. Toxic effects of excess nickel were observed through slower growth and biomass decrease, together with increased reactive oxygen species (ROS) production and lipid peroxidation. Nickel exposure decreased chlorophyll a, b, total chlorophyll as well as carotenoid concentration. Addition of sodium benzoate, potent ROS scavenger, showed concentration-dependent disturbing effect on nickel hyperaccumulation, lowering the content of accumulated nickel in A. markgrafii shoots. General reducing power represented by low molecular weight antioxidants and phenols was inversely correlated with nickel concentration. Among the investigated antioxidative enzymes, POD seems to play important role in ROS level regulation upon excessive nickel in medium.
ISSN:0137-5881
1861-1664
DOI:10.1007/s11738-012-1001-3