Lupine embryo axes under salinity stress. I. Ultrastructural response

Embryonic root is the primary site of salinity perception in germinating seeds. To understand better the NaCl stress response of lupine embryo axes, ultrastructural approach combined with analysis of DNA degradation was used. In this study lupine embryo axes were cultured in vitro on the medium supp...

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Bibliographic Details
Published in:Acta physiologiae plantarum 2013-07, Vol.35 (7), p.2219-2228
Main Authors: Wojtyla, Łukasz, Rucińska-Sobkowiak, Renata, Kubala, Szymon, Garnczarska, Małgorzata
Format: Article
Language:English
Subjects:
Agriculture
apoptosis
Biomedical and Life Sciences
DNA
DNA damage
DNA fragmentation
electrophoresis
endoplasmic reticulum
Life Sciences
Lupinus luteus
Original Paper
Plant Anatomy/Development
Plant Biochemistry
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
salinity
salt concentration
salt stress
seeds
sodium chloride
starch granules
stress response
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Summary:Embryonic root is the primary site of salinity perception in germinating seeds. To understand better the NaCl stress response of lupine embryo axes, ultrastructural approach combined with analysis of DNA degradation was used. In this study lupine embryo axes were cultured in vitro on the medium supplemented with two salt concentrations 250 and 500 mM to differ the reaction. To assess the rate of DNA damage, alkaline electrophoresis of isolated nuclei and DNA fragmentation analysis were performed. Results of these studies suggest programmed cell death induction under salinity stress. Moreover, ultrastructure observations revealed other characteristic features of programmed cell death like endoplasmic reticulum reorganization, increased level of vacuolization, chromatin condensation and starch grains degradation. Our comparative analysis of ultrastructure changes and DNA fragmentation speak in favour of programmed cell death in lupine (Lupinus luteus L. ‘Mister’) embryo axes treated for 12 h with 250 and 500 mM NaCl.
ISSN:0137-5881
1861-1664
DOI:10.1007/s11738-013-1258-1