Evaluation of salinity tolerance in seedlings of sugar beet (Beta vulgaris L.) cultivars using proline, soluble sugars and cation accumulation criteria

• Published in: Acta physiologiae plantarum 2013-09, Vol.35 (9), p.2665-2674
• Main Authors: Wu, Guo-Qiang, Liang, Na, Feng, Rui-Jun, Zhang, Jing-Jing
• Format: Article
• Language: English
• Subjects: Agriculture; Beta vulgaris; Biomedical and Life Sciences; calcium; cultivars; Life Sciences; Original Paper; Plant Anatomy/Development; Plant Biochemistry; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; proline; roots; salinity; salt stress; salt tolerance; seedlings; shoots; sodium; sodium chloride; sugar beet; sugars
• ISSN: 0137-5881; 1861-1664
• DOI: 10.1007/s11738-013-1298-6

Salinity tolerance of sugar beet (Beta vulgaris L.) cultivars in terms of growth, proline and soluble sugars concentrations, and Na⁺/K⁺ and Na⁺/Ca²⁺ ratios were analyzed in this study. Three-week-old seedlings of three sugar beet cultivars, ‘Gantang7’, ‘SD13829’, and ‘ST21916’, differing in salinity tolerance, were treated with 0, 50, 100, and 200 mM NaCl. Plant shoots and roots were harvested at 7 days after treatment and subjected to analysis. Low concentration of NaCl (50 mM) enhanced fresh and dry weights of shoot and root in ‘Gantang7’, whereas high one (200 mM) reduced growth in all cultivars and the less reduction was observed in ‘ST21916’. Shoot proline was strongly induced by salinity stress in both ‘Gantang7’ and ‘SD13829’, while it remained unchanged in ‘ST21916’. The addition of 50 mM NaCl significantly increased shoot soluble sugars concentrations in ‘Gantang7’ while it had no significant effects in the other two cultivars. ‘Gantang7’ also showed a higher level of root soluble sugars concentration as compared to the other two cultivars. At 50 mM NaCl, the lower shoot Na⁺ concentration, and the higher shoot K⁺ and root Ca²⁺ concentration in ‘Gantang7’ resulted in the lower shoot Na⁺/K⁺ and root Na⁺/Ca²⁺ ratio. However, ‘SD13829’ maintained a lower Na⁺/K⁺ ratio in both shoot and root when subjected to 200 mM NaCl treatment. According to comprehensive evaluation on salinity tolerance, it is clear that ‘Gantang7’ is more tolerant to salinity than the other two cultivars. Therefore, it is suggested that ‘Gantang7’ should be more suitable for cultivating in the arid and semi-arid irrigated regions.