RsERF1 derived from wild radish (Raphanus sativus) confers salt stress tolerance in Arabidopsis

The change in environmental parameters affects normal growth of plants, eventually reduces agricultural production. Ethylene plays vital roles in plant stress responses, germination, fruit ripening, organ abscission, pathogen response, and senescence. Expression of an ethylene-responsive transcripti...

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Bibliographic Details
Published in:Acta physiologiae plantarum 2014-04, Vol.36 (4), p.993-1008
Main Authors: Ayarpadikannan, Selvam, Chung, Eunsook, Kim, Kyoungmi, So, Hyun-Ah, Schraufnagle, Kenneth Ryan, Lee, Jai-Heon
Format: Article
Language:English
Subjects:
abscission
Agriculture
Arabidopsis
Biomedical and Life Sciences
cDNA libraries
complementary DNA
ethylene
gene overexpression
genetically modified organisms
germination
halophytes
Life Sciences
Original Paper
pathogens
Plant Anatomy/Development
Plant Biochemistry
Plant Genetics and Genomics
plant growth
Plant Pathology
Plant Physiology
post-translational modification
radishes
Raphanus sativus
reporter genes
ripening
salt stress
senescence
sodium chloride
stress tolerance
transcription factors
transcriptional activation
yeasts
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Summary:The change in environmental parameters affects normal growth of plants, eventually reduces agricultural production. Ethylene plays vital roles in plant stress responses, germination, fruit ripening, organ abscission, pathogen response, and senescence. Expression of an ethylene-responsive transcription factor (ERF) was induced in Korean halophyte, Raphanus sativus var. hortensis f. raphanistroides (wild radish) by 200-mM sodium chloride (NaCl). Raphanus sativus ethylene-responsive transcription factor 1 (RsERF1) is also localized to nucleus, similar to other transcription factors. In yeast, RsERF1 showed transcriptional activation property, by expressing the reporter gene. Being a TF, RsERF1 specifically bound to the cis-acting elements, GCC box and DRE/CRT in vitro, to initiate transcription. Homozygous T3 transgenic Arabidopsis, overexpressing RsERF1, showed significant tolerance against salt stress in soil-grown conditions. The tolerance was also marked by an increased germination rate of RsERF1 transgenics in salt-containing media. In RsERF1 overexpression lines, abiotic stress-related genes such as ABF3, ABF4, ADH, Rab18, and SUS1 were upregulated by 200-mM NaCl. ERFs have been studied and proven for their tolerance potential against various abiotic stresses, but RsERF1 belongs to an ERF subgroup called ethylene-responsive transcription factor related to AP2 (ERF-RAP2). Thus, this is a first report for ERF-RAP2 from Korean halophyte cDNA library. We believe that extensive posttranslational modification studies will reveal the role and location of RsERF1 in stress tolerance pathway.
ISSN:0137-5881
1861-1664
DOI:10.1007/s11738-013-1478-4