Hydroxycinnamates from elecampane (Inula helenium L.) callus culture

Hydroalcoholic extract from Inula helenium callus tissue showed remarkable reducing capacity. An HPLC/DAD analysis revealed the presence of numerous hydroxycinnamic acid derivatives, including chlorogenic acid (5-O-CQA, 0.1 % dry weight) and 3,5-dicaffeoylquinic acid (3,5-DCQA, 0.3 % dry weight), wh...

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Bibliographic Details
Published in:Acta physiologiae plantarum 2016-02, Vol.38 (2), Article 41
Main Authors: Stojakowska, Anna, Malarz, Janusz, Kiss, Anna K
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
caffeic acid
callus
callus culture
chlorogenic acid
high performance liquid chromatography
Inula helenium
Life Sciences
Plant Anatomy/Development
Plant Biochemistry
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Short Communication
tissue culture
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Summary:Hydroalcoholic extract from Inula helenium callus tissue showed remarkable reducing capacity. An HPLC/DAD analysis revealed the presence of numerous hydroxycinnamic acid derivatives, including chlorogenic acid (5-O-CQA, 0.1 % dry weight) and 3,5-dicaffeoylquinic acid (3,5-DCQA, 0.3 % dry weight), which were among major constituents of the examined extract. Application of a hyphenated chromatographic method—UHPLC/DAD/MSⁿ—allowed identification of sixteen compounds, derivatives of caffeic acid. Apart from the compounds commonly found in Inula sp., the plant material contained eight conjugates of caffeic acid with aldaric acid. The aldarates constituted over 50 % of the hydroxycinnamate fraction in the examined tissue. This is the first report on the occurrence of the caffeoylaldaric acids in Inula sp. and in a plant tissue culture.
ISSN:0137-5881
1861-1664
DOI:10.1007/s11738-016-2069-y