Enzymatic detection of ethanol based on H2O2-sensitive quantum dots

A rapid and sensitive fluorometric method for the enzymatic detection of ethanol using CdSe/ZnS quantum dots (QDs) is proposed. The photoluminescence of QDs is sensitive to H 2 O 2 . This finding leads to a novel approach for the determination of ethanol using alcohol oxidase (AO x ) which, on oxida...

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Bibliographic Details
Published in:Journal of Central South University 2012-11, Vol.19 (11), p.3040-3045
Main Authors: Fu, Xin, Zhang, He, Xiao, Jun-ping, Liu, Su-qin
Format: Article
Language:English
Subjects:
Engineering
Metallic Materials
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Summary:A rapid and sensitive fluorometric method for the enzymatic detection of ethanol using CdSe/ZnS quantum dots (QDs) is proposed. The photoluminescence of QDs is sensitive to H 2 O 2 . This finding leads to a novel approach for the determination of ethanol using alcohol oxidase (AO x ) which, on oxidation of ethanol, produces H 2 O 2 . The method has higher sensitivity, wider analytical range (0.1–8 mmol/L), and a lower detection limit (0.05 mmol/L). The relationship between quenching of the photoluminescence of the QDs and the concentration of ethanol is linear.
ISSN:2095-2899
2227-5223
DOI:10.1007/s11771-012-1376-8