Chiral ionic liquids as chiral selectors for separation of tryptophan enantiomers by ligand exchange chromatography

Chiral ionic liquids (CILs) containing imidazolium cations and L -Proline ( L -Pro) anions were applied as chiral selector to separate tryptophan (Trp) enantiomers on a C18 column by ligand exchange chromatography. Several factors influencing Trp enantiomers separation, such as alkyl chain length of...

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Bibliographic Details
Published in:Journal of Central South University 2013-05, Vol.20 (5), p.1173-1177
Main Authors: Yang, Yan-xia, Li, Jing, Jiang, Xin-yu
Format: Article
Language:English
Subjects:
Engineering
Metallic Materials
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Summary:Chiral ionic liquids (CILs) containing imidazolium cations and L -Proline ( L -Pro) anions were applied as chiral selector to separate tryptophan (Trp) enantiomers on a C18 column by ligand exchange chromatography. Several factors influencing Trp enantiomers separation, such as alkyl chain length of CILs, concentrations of Cu 2+ and CILs, pH of the mobile phase, flow rate, organic solvent and temperature, were studied. Under the optimal conditions, the Trp enantiomers could be successfully separated within 21 min with the resolution of 2.30. At the same time, some thermodynamical parameters were obtained. The experimental results show that the enthalpy values of the Trp enantiomers are negative, indicating that the separation process is exothermic. And the enthalpy values of D -Trp are larger than those of L -Trp, which indicates that L -Trp could form more stable ternary complexes with tryptophan enantiomers.
ISSN:2095-2899
2227-5223
DOI:10.1007/s11771-013-1600-1